StickSpongesCollect™ Cellulose D/E Neutralizing Broth, 10 mL

Microbial Sampling Tool for surface sampling, using a plastic stick with an attached cellulose sponge moistened with D/E Neutralizing Broth to neutralize the effects of residual disinfectants and protect microorganisms during transport and analysis
Code: 4107023
Packaging: 100 pieces/box or customer request.
Storage Conditions: 4 – 25°C.
Transportation: Ambient temperature.
Shelf Life: 24 months from the date of manufacture.

INTENDED USE

StickSpongesCollect™ Cellulose D/E Neutralizing Broth, 10 mL is designed for microbial sampling from surfaces in food processing, medical, and pharmaceutical environments. The primary purpose of this product is to facilitate the collection of microbial samples from surfaces, particularly from hard-to-reach areas such as drains, equipment, and surrounding areas in food production facilities. D/E Neutralizing Broth helps neutralize the effects of disinfectants and protects microorganisms throughout transport and analysis.

PRINCIPLES

StickSpongesCollect™ Cellulose D/E Neutralizing Broth, 10 mL utilizes a plastic stick with an attached cellulose sponge combined with 10 mL of D/E Neutralizing Broth to collect microorganisms from surfaces. D/E Neutralizing Broth neutralizes the effects of disinfectants such as chlorine, quaternary ammonium compounds, iodine, phenolic compounds, mercury, formaldehyde, and glutaraldehyde, protecting microorganisms from their harmful effects. This medium provides the necessary nutrients to recover and promote microorganism growth, while maintaining their viability during transport. D/E Neutralizing Broth not only neutralizes disinfectants but also preserves the integrity of microorganisms, ensuring accurate analysis results. Bromocresol purple is included in the medium as a pH indicator, allowing monitoring of the neutralization process.

Inspect the StickSpongesCollect™ product to ensure there is no damage or contamination during storage.
Write sample information on the provided label attached to the product packaging.
Tear the bag along the pre-marked perforated line.
Remove the StickSpongesCollect™ stick by pulling the bag from the outside. Be careful not to touch the inside of the bag.
Rub the StickSpongesCollect™ sponge on the area to be sampled.
After sampling, return the sampling stick to the bag, making sure not to insert it too deep to avoid touching the inside of the bag.
Remove the sponge from the stick by holding the sponge from the outside of the bag and gently shaking until the sponge comes off the stick.
Use the provided tie to seal the StickSpongesCollect™ bag by rolling the top of the bag and tightly twisting the tie inside the bag..
The sample must be sent to the laboratory as soon as possible to prevent changes in microbial characteristics. The transportation process should comply with sample storage regulations.

Growth Promotion Test: ≤ 10³ CFU/mL microorganisms
Control strain	Incubation Conditions	Specifications
E. coli ATCC 25922	24 – 48 h at 35 – 37°C	Good growth
S. aureus ATCC 25923	24 – 48 h at 35 – 37°C	Good growth
S. typhimurium ATCC 14028	24 – 48 h at 35 – 37°C	Good growth
Sterility control	No bacterial growth after incubation at 35 – 37°C for 72 hrs
Appearance	The solution contains a homogeneous sponges, without any stratification, purple, cloudy.

Dextrose………………………………………………………….. 10,0 g

Sodium bisulfite………………………………………………… 2,5 g

Lecithin……………………………………………………………. 7,0 g

Yeast extract…………………………………………………….. 2,5 g

Sodium thiosulfate…………………………………………….. 6,0 g

Sodium thioglycollate………………………………………… 1,0 g

Casein peptone…………………………………………………. 5,0 g

Brom cresol purple…………………………………………….. 0,02 g

Polysorbate 80 (Tween 80)…………………………………. 5,0 g

pH of the ready-to-use medium at 25°C: 7,6 ± 0,2

* Adjusted and /or supplemented as required to meet performance criteria

Product Name	Code	Packaging
StickSpongesCollect™ Cellulose D/E Neutralizing Broth, 10 mL	4107023	100 pieces/box or customer request.