StickSpongesCollect™ Polyurethane Buffered Peptone Water, 10 mL

Microbial Sampling Tool for surface sampling, using a plastic stick with an attached polyurethane sponge impregnated with Buffered Peptone Water medium to recover and protect microorganisms during transportation and analysis.
Code: 4107014
Packaging: 100 pieces/box or customer request.
Storage Conditions: 4 – 25°C.
Transportation: Ambient temperature.
Shelf Life: 24 months from the date of manufacture.

INTENDED USE

StickSpongesCollect™ Polyurethane Buffered Peptone Water, 10 mL is designed for microbial sampling from surfaces in food processing, medical, and pharmaceutical environments. The primary purpose of this product is to facilitate the collection of microbial samples from surfaces, particularly from hard-to-reach areas such as drains, equipment, and surrounding areas in food production facilities. Buffered Peptone Water is a non-selective pre-enrichment medium for Salmonella species in food, helping to maintain the viability and stability of microorganisms during transportation and analysis.

PRINCIPLES

StickSpongesCollect™ Polyurethane Buffered Peptone Water, 10 mL utilizes a plastic stick with an attached biocide-free polyurethane sponge combined with 10 mL of Buffered Peptone Water to collect microorganisms from surfaces. Buffered Peptone Water contains key components such as peptone, sodium chloride (NaCl), and phosphate buffer. Peptone provides a source of nitrogen and amino acids essential for bacterial growth, aiding in the recovery of microorganisms weakened during food processing. Sodium chloride (NaCl) maintains osmotic pressure, creating a favorable environment for microorganisms, while the phosphate buffer helps stabilize the pH, ensuring an optimal environment for bacterial recovery and growth before being transferred to selective media. This process helps protect microorganisms throughout transportation and analysis without altering the sample’s characteristics.

Inspect the StickSpongesCollect™ product to ensure there is no damage or contamination during storage.
Write sample information on the provided label attached to the product packaging.
Wear the provided sterile gloves (if available).
Tear the bag along the pre-marked perforated line.
Remove the StickSpongesCollect™ stick by pulling the bag from the outside. Be careful not to touch the inside of the bag.
Rub the StickSpongesCollect™ sponge on the area to be sampled.
After sampling, return the sampling stick to the bag, making sure not to insert it too deep to avoid touching the inside of the bag.
Remove the sponge from the stick by holding the sponge from the outside of the bag and gently shaking until the sponge comes off the stick.
Use the provided tie to seal the StickSpongesCollect™ bag by rolling the top of the bag and tightly twisting the tie inside the bag..
The sample must be sent to the laboratory as soon as possible to prevent changes in microbial characteristics. The transportation process should comply with sample storage regulations.

Growth Promotion Test: ≤ 10³ CFU/mL microorganisms
Control strain	Incubation Conditions	Specifications
S. typhimurium ATCC 14028	18 – 24 h at 35 – 37°C	Good growth
S.aureus ATCC 25923	18 – 24 h at 35 – 37°C	Good growth
E. coli ATCC 25922	18 – 24 h at 35 – 37°C	Good growth
Sterility control	No bacterial growth after incubation at 35 – 37°C for 72 hrs
Appearance	The solution contains a homogeneous sponges, without any stratification, light yellow, clear.

Peptone……………………………………………………………. 10,0 g

Sodium Choloride……………………………………………… 5,0 g

Disodium phosphate, anhydrous………………………….. 3,57 g

Monopotassium phosphate, anhydrous…………………. 1,5 g

pH of the ready-to-use medium at 25°C: 7,2 ± 0,2

* Adjusted and /or supplemented as required to meet performance criteria

Product Name	Code	Packaging
StickSpongesCollect™ Polyurethane Buffered Peptone Water, 10 mL	4107014	100 pieces/box (5 bags) or customer request.