StickSpongesCollect™ Polyurethane Letheen Broth, 10 mL

Microbial Sampling Tool for surface sampling, using a plastic stick with an attached polyurethane sponge moistened with Letheen Broth to neutralize the effects of disinfectants and protect microorganisms during transport and analysis
Code: 4107016
Packaging: 100 pieces/box or customer request.
Storage Conditions: 4 – 25°C.
Transportation: Ambient temperature.
Shelf Life: 24 months from the date of manufacture.

INTENDED USE

StickSpongesCollect™ Polyurethane Letheen Broth, 10 mL is designed for microbial sampling from surfaces in food processing, medical, and pharmaceutical environments. The primary purpose of this product is to facilitate the collection of microbial samples from surfaces, particularly from hard-to-reach areas such as drains, equipment, and surrounding areas in food production facilities. Letheen Broth is a nutrient-rich pre-enrichment medium that helps test the effectiveness of disinfection processes while maintaining the viability and stability of microorganisms during transport and analysis.

PRINCIPLES

StickSpongesCollect™ Polyurethane Letheen Broth, 10 mL utilizes a plastic stick with an attached biocide-free polyurethane sponge combined with 10 mL of Letheen Broth to collect microorganisms from surfaces. Letheen Broth is a nutrient-rich medium containing lecithin and Tween 80. Lecithin neutralizes quaternary ammonium compounds, a key component in disinfectants, ensuring that microorganisms are not affected by residual disinfectants on surfaces. Tween 80 neutralizes disinfectants such as phenol, hexachlorophene, and formalin, protecting microorganisms from their harmful effects. This process helps recover and maintain the stability of microorganisms in food processing environments, ensuring they grow well throughout transport and analysis before being transferred to selective media without any alteration of their characteristics.

Inspect the StickSpongesCollect™ product to ensure there is no damage or contamination during storage.
Write sample information on the provided label attached to the product packaging.
Tear the bag along the pre-marked perforated line.
Remove the StickSpongesCollect™ stick by pulling the bag from the outside. Be careful not to touch the inside of the bag.
Rub the StickSpongesCollect™ sponge on the area to be sampled.
After sampling, return the sampling stick to the bag, making sure not to insert it too deep to avoid touching the inside of the bag.
Remove the sponge from the stick by holding the sponge from the outside of the bag and gently shaking until the sponge comes off the stick.
Use the provided tie to seal the StickSpongesCollect™ bag by rolling the top of the bag and tightly twisting the tie inside the bag..
The sample must be sent to the laboratory as soon as possible to prevent changes in microbial characteristics. The transportation process should comply with sample storage regulations

Growth Promotion Test: ≤ 10³ CFU/mL microorganisms
Control strain	Incubation Conditions	Specifications
E. coli ATCC 25922	24 – 48 h at 35 – 37°C	Good growth
S. aureus ATCC 25923	24 – 48 h at 35 – 37°C	Good growth
S. typhimurium ATCC 14028	24 – 48 h at 35 – 37°C	Good growth
Sterility control	No bacterial growth after incubation at 35 – 37°C for 72 hrs
Appearance	The solution contains a homogeneous sponges, without any stratification, light yellow, clear.

Casein peptone…………………………………………………. 5,0 g

Beef extract……………………………………………………… 5,0 g

Polysorbate 80 (Tween 80)…………………………………. 5,0 g

Lecithin……………………………………………………………. 0,7 g

Meat peptone……………………………………………………. 20,0 g

Sodium bisulfite………………………………………………… 0,1 g

Sodium chloride………………………………………………… 5,0 g

Yeast extract…………………………………………………….. 2,0 g

pH of the ready-to-use medium at 25°C: 7,2 ± 0,2

* Adjusted and /or supplemented as required to meet performance criteria

Product Name	Code	Packaging
StickSpongesCollect™ Polyurethane Letheen Broth, 10 mL	4107016	100 pieces/box or customer request.