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SwabCollect™ Half Fraser Broth, 5 mL
- Half-Fraiser Broth is used for the selective and differential (primary enrichment broth) enrichment of Listeria monocytogenes and of Listeria spp in food products.
- Code: 4108027
- Packaging: 50 set/box or customer request.
- Storage Conditions: 4 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 18 months from the date of manufacture.
INTENDED USE
- SwabCollect™ Half Fraser Broth, 5 mL is designed for microbiological sampling from surfaces and hard-to-reach areas in food processing, healthcare, and pharmaceutical environments. The product aids in collecting samples from narrow or difficult-to-reach areas, ensuring that microorganisms are protected throughout the transport and analysis process. Half Fraser Broth is a primary enrichment medium for the detection of Listeria spp. in environmental and processing area samples.
PRINCIPLES
- SwabCollect™ Half Fraser Broth, 5 mL combines a sterile swab with a plastic tube containing 5 mL of Half Fraser Broth to collect microorganisms from surfaces. Half Fraser Broth is a semi-selective enrichment medium developed to recover and promote the growth of stressed, injured, or low-level Listeria cells that may remain after surface cleaning or disinfection. The medium supports the recovery of Listeria while inhibiting competing background flora, thereby enhancing the sensitivity and reliability of pathogen detection in environmental monitoring programs
- Write the sample information on the pre-labeled sticker on the plastic tube containing the solution.
- Carefully open the cap of the solution tube to avoid contaminating the internal environment.
- Immerse the swab tip into the solution in the tube, ensuring the swab tip is completely moistened with the solution.
- Collect the sample by gently rubbing the swab on the area to be sampled. Be careful not to touch the swab tip with your hands.
- Insert the swab back into the tube containing the solution.
- Break off the excess swab tip to fit the tube.
- Close the plastic tube tightly to protect the sample and prevent contamination during transport.
- Transport the sample immediately to the laboratory to ensure the stability of the microorganisms, following sample storage guidelines..
- SwabCollect™ Half Fraser Broth, 5 mL is produced in a closed environment, adhering to strict hygiene regulations. The plastic tube and swab are sterilized using gamma irradiation, ensuring no microorganisms affect the quality of the sample.
- BacterLab ensures the quality of each product batch of the medium by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| L. monocytogenes ATCC 13932 | 41,5°C for 18 – 24 hours | Good growth |
| E. coli ATCC 35218 | Good growth |
1. Tools
- Sterile swab
- Sterile plastic tube, size 16 x 100 mm
2. Medium
- Volume of medium in the plastic tube: 5 mL
- Components of the medium:
For 1 liter of medium
| Peptone | 20,0 g |
| Yeast extract | 5,0 g |
| Glucose | 20,0 g |
| Tween 80 | 1,08 g |
| Dipotassium phosphate | 2,0 g |
| Sodium | 5,0 g |
| Ammonium citrate | 2,0 g |
| Magnesium sulfate | 0,2 g |
| Manganese sulfate | 0,05g |
pH of the ready-to-use medium at 25°C: 7,2 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| SwabCollect™ Half Fraser Broth, 5 mL | 4108027 | 50 set/box or customer request |
| Each kit includes: one plastic tube with sample transport medium and one sterile swab. |
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