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BacterPlate™ GNR/STAPH/STREP 3-Compartment Medium
- Ready-to-use medium on 90mm plates for used to culture and test microorganisms.
- Code: 05053
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterPlate™ GNR/STAPH/STREP 3-compartment medium is recommended for culturing and examining pathogenic microorganisms in medical, clinical, food, and cosmetic applications.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterPlate™ GNR/STAPH/STREP 3-compartment mediumconsists of three compartments with specific culture media:
- GNR Compartment: Contains chromogenic Mastitis (GN) medium for isolating and differentiating Gram-negative pathogens such as:
- pneumoniae: Blue-gray colonies
- coli: Pink to dark red colonies
- aeruginosa: Transparent colonies
- Other microorganisms are inhibited.
- STAPH Compartment: Contains chromogenic Staphylococcus medium for directly isolating and differentiating Staphylococcus:
- aureus: Pink to lavender colonies
- saprophyticus: Turquoise colonies
- Other microorganisms are inhibited.
- STREP Compartment: Contains chromogenic Streptococcus medium for isolating and differentiating Streptococcus:
- agalactiae: Blue colonies
- uberis: Blue-gray colonies
- Enterococcus: Light to dark purple colonies
- Other microorganisms are inhibited.
- GNR Compartment: Contains chromogenic Mastitis (GN) medium for isolating and differentiating Gram-negative pathogens such as:
- Allow the culture plate to reach room temperature.
- Dry the plate in an incubator by slightly opening the lid.
- Inoculate the sample directly onto the medium surface for isolation.
- Incubate aerobically at 35 – 37°C for 18 – 24 hours (extend if necessary).
- Observe colony morphology and reactions (if any).
RESULTS
Observe growth characteristics such as colony morphology, color and size.
- GNR Compartment
| Microorganism | Colony Appearance |
| E. coli | Pink to dark red |
| Klebsiella, Enterobacter, Citrobacter | Metallic blue (+/- red halo) |
| Proteus, Morganella, Providencia | Brown halo |
| Pseudomonas | Transparent (+/- blue to green) |
| Candida albicans | Cream-colored, pointed colonies |
| Gram (+) bacteria | Inhibited |
- STAPH Compartment
| Microorganism | Colony Appearance |
| S. aureus | Pink to lavender |
| S. epidermidis | Colorless to light pink |
| S. saprophyticus | Blue-gray |
| Staphylococci khác | Varying colors |
| Streptococci | Inhibited |
| Gram (-) bacteria | Inhibited |
- STREP Compartment
| Microorganism | Colony Appearance |
| S. agalactiae | Blue |
| S. uberis | Blue to metallic blue |
| Enterococcus | Light to dark purple |
| Other Streptococcus | Varying colors |
| Other Gram (+) bacteria | Inhibited |
| Gram (-) bacteria | Inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation Conditions | Expected results |
| GNR Compartment | ||
| K. pneumoniae ATCC 13883 |
35 – 370C, 18 – 24 hours |
Good growth, blue-gray colonies |
| Escherichia coli ATCC 25922 | Good growth, light red colonies | |
| P. aeruginosa ATCC 10145 | Good growth, transparent colonies | |
| S. aureus ATCC 25923 | No growth, inhibited | |
| E. faecalis ATCC 29212 | No growth, inhibited | |
| STAPH Compartment | ||
| S. aureus ATCC 25923 | 35 – 370C, 18 – 24 hours | Good growth, lavender colonies |
| S. saprophyticus ATCC 15305 | Good growth, turquoise colonies | |
| Escherichia coli ATCC 25922 | No growth, inhibited | |
| C. albicans ATCC 10231 | No growth, inhibited | |
| E. faecalis ATCC 29212 | No growth, inhibited | |
| STREP Compartment | ||
| S. agalactiae ATCC 13813 | 35 – 370C, 18 – 24 hours | Good growth, blue colonies |
| S. uberis ATCC 700407 | Good growth, blue-gray colonies | |
| E. faecalis ATCC 29212 | Good growth, purple colonies | |
| Escherichia coli ATCC 25922 | No growth, inhibited | |
| C. albicans ATCC 10231 | No growth, inhibited | |
| S. aureus ATCC 25923 | No growth, inhibited | |
- GNR Compartment (per liter, reference values)
| Agar | 15,0 g |
| Peptone and yeast extract | 17,0 g |
| Chromogenic mix | 1,2 g |
pH of the ready-to-use medium at 250C: 7,4 ± 0,2
- STAPH Compartment (per liter, reference values)
| Agar | 15,0 g |
| Peptone and yeast extract | 40,0 g |
| Salts | 30,0 g |
| Chromogenic and selective mix | 2,4 g |
pH of the ready-to-use medium at 250C: 7,3 ± 0,2
- STREP Compartment (per liter, reference values)
| Agar | 15,0 g |
| Peptones and growth factors | 20,0 g |
| Salts | 7,5 g |
| Chromogenic and selective mix | 2,4 g |
| Growth factors | 8,0 mL |
pH of the ready-to-use medium at 25°C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ GNR/STAPH/STREP 3-Compartment Medium | 05053 | 10 plates/ box or as per customer request. |
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