BacterChrom™ TBX Plates

Ready-to-use chromogenic medium on 90mm plates for the detection the detection and enumeration of Escherichia coli in foods.
Code: 01045
Packaging: 10 plates/box or as per customer requirem ents.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterChrom™ TBX is based on Tryptone Bile Salts Agar medium, used to detect and enumerate E. coli in foods, with the addition of a chromogenic agent, x-ß-D-Glucuronide, to detect the presence of the enzyme glucuronidase, which is highly specific for E. coli.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

The released chromophore in BacterChrom™ TBX is colored and target colonies are easily identified. E. coli absorbs the chromogenic agent x-ß-D-glucuronide, and the intracellular glucuronidase enzyme activity breaks the bond between the chromophore and the glucuronide. The released chromophore is colored and builds up within the cells, causing the E. coli colonies to be blue-green colored.
Casein peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Bile Salts are inhibitors to other Gram-positive organisms and suppress coliform bacteria. Bacteriological agar is the solidifying agent.
ISO 16649 specifies a horizontal method for the enumeration of ß-glucuronidase-positive E. coli in products intended for human consumption or for the feeding of animals.
The negative b-ß-glucuronidase E. coli colonies are colorless, e.g. E. coli O157: H7. The high temperatures (44°C) inhibit the growth of E. coli O157: H7.

Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
Inoculate the BacterChrom™ TBX either by the plating method in depth, seeding on the surface or by the membrane filtration method.
Incubate the plates for 21 hours at a temperature of 44°C.

RESULTS

Calculate the number of positive Escherichia coli ß-glucuronidase colonies from the number of typical blue colonies.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

For 1 liter of medium

Casein enzymatic digest	20,0 g
Bile salts n°3	1,5 g
BCIG (5-bromo-4-chloro-3-indolyl β-D-glucuronate)	0,075
Bacteriological agar	15,0 g

pH of the ready-to-use medium at 25°C: 7,2 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterChrom™ TBX	01045	10 plates/box or as per customer request.