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BacterChrom™ Y.enterocolitica Plates
- Ready-to-use chromogenic medium on 90mm plates for the detection and differentiation of pathogenic Yersinia enterocolitica strains
- Code: 01038
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ Y.enterocolitica is a selective chromogenic culture medium designed for the direct qualitative detection, differentiation, and presumptive identification of pathogenic biotypes of Yersinia enterocolitica.
- The medium can also be applied in food and environmental analyses for the detection of Y. enterocolitica in products such as recreational water, livestock, and raw or processed foods.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ Y.enterocolitica utilizes chromogenic substrates that interact with specific enzymatic activities of target bacteria, producing distinct colony colors for differentiation. The medium incorporates selective agents to inhibit the growth of non-target organisms, ensuring high specificity. This approach enhances the accuracy of detection and simplifies the presumptive identification process.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Direct streaking sample onto the plate
- Incubate in aerobic conditions at 30± 2°C for 36 – 48h.
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| Pathogenic Y. enterocolitica | mauve |
| Yersinia spp | metallic blue or inhibited |
| Other Enterobacteriacae | metallic blue or inhibited |
| Gram (+) bacteria | inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Y. enterocolitica pYV+ ATCC 23715 | Incubate for 36 – 48 hours at 30 ± 2°C | Mauve colonies |
| E. coli ATCC 35218 | Inhibited | |
| E. faecalis ATCC 29212 | Inhibited |
For 1 liter of medium
| Peptone | 20,0 g |
| Chromogenic mix | 1,3 g |
| Salt | 5,0 g |
| Agar | 15,0 g |
| Selective mix (supplement) | 0,1 g |
pH of the ready-to-use medium at 25°C: 7,0 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ Y.enterocolitica | 01038 | 10 plates/box or as per customer request. |
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