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BacterChrom™ CCA Plates
- Ready-to-use chromogenic medium on 90mm plates for the detection and enumeration of E. coli and coliform bacteria in water according to the ISO 9308-1 norm.
- Code: 01044
- Packaging: 10 plates/box or as per customer requirem ents.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ CCA is a selective chromogenic medium designed for the detection and enumeration of ß-glucuronidase-positive E. coli and coliforms in water samples with low bacterial background flora. It is particularly suited for applications requiring compliance with ISO 9308-1 standards, ensuring safe water quality monitoring for human consumption, including drinking water, pool water, and treated water samples.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- Coliforms, Enterobacteriacae able to ferment lactose (lactose positive Enterobacteriacae), are bacteria present not only in human and warm blooded animals intestinal flora but also in the soil and water. Coliforms are proof of organic, environmental or faecal contamination. Faecal contamination, due to coliforms coming from animal waste, consists mainly of Escherichia coli and thermotolerant Klebsiella. Strict regulations exist for E. coli/coliform presence in water samples. This can be explained by the importance of these germs in determining drinking water safety and process efficiency of treatment, storage and distribution.
- BacterChrom™ CCA contains chromogenic substrates that interact with specific enzymatic activities of target organisms, producing distinct colony colors for differentiation. Selective agents are incorporated to suppress non-target organisms, ensuring high specificity and accuracy. This formulation simplifies the identification and enumeration of coliforms and E. coli, supporting efficient workflows in microbiological
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Asseptically filter the water sample through a 0.45 μm nitrocellulosic (or equivalent) membrane.
- Place the membrane on the BacterChrom™ CCA
- Turn upside down the plate and incubate in aerobic conditions at 36 ± 2°C for 18 – 24 hours
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| E. coli | metallic blue to violet |
| Other coliform bacteria | pink to red |
| Other Gram (-) bacteria | colorless |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| P. aeruginosa ATCC 9027 | Incubate for 24 hours at 37°C | Good growth |
| K. aerogenes ATCC 13048 | PR ³ 70% | |
| E. coli ATCC 35218 | PR ³ 70% |
For 1 liter of medium
| Peptone yeast | 3,0 g |
| Sodium dihydrogen phosphate | 2,2 g |
| Agar | 14,9 g |
| NaCl | 5,0 g |
| Disodium hydrogen phosphate | 2,7 g |
| Sodium pyruvate | 1,0 g |
| Tryptophan | 1,0 g |
| Sorbitol | 1,0 g |
| Tergitol | 0,15 g |
| Chromogenic mix | 0,5 g |
pH of the ready-to-use medium at 25°C: 6,8 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ CCA | 01044 | 10 plates/box or as per customer request. |
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