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BacterChrom™ Cronobacter Plates
- Ready-to-use chromogenic medium on 90mm plates for isolating Cronobacter bacteria according to ISO 22964 standards.
- Code: 01043
- Packaging: 10 plates/box or as per customer requirem ents.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ Cronobacter is a selective chromogenic medium designed for the isolation and detection of Cronobacter species. It is particularly useful for testing food, animal feed, and environmental samples, in compliance with ISO 22964 standards. This medium supports rapid and reliable screening for Cronobacter spp., aiding in food safety and quality control processes.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ Cronobacter contains chromogenic substrates that interact with specific enzymatic activities of Cronobacter spp., producing green to blue colonies for easy identification. Selective agents suppress non-target organisms, ensuring high specificity. This formulation facilitates efficient isolation and differentiation of Cronobacter spp. from other Gram-negative bacteria and contaminants.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- From an appropriate enrichment broth, streak a loopful (10 µL) onto plate.
- Incubate at 41.5 ± 1°C for 24 h ± 2 h, in aerobic conditions
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| Cronobacter spp. | green to blue |
| Other Gram (-) bacteria | colorless, clear green, black, yellow |
| Gram (+) bacteria | inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| C. sakazakii ATCC 29544 | Incubate for 24 hours at 41,5°C | Green colonies |
| E. coli ATCC 35218 | Colorless colonies | |
| S. aureus ATCC 25923 | Inhibited | |
| E. faecalis ATCC 29212 | Inhibited |
For 1 liter of medium
| Casein Peptone | 7,0 g |
| Yeast extract | 3,0 g |
| Agar | 15,3 g |
| NaCl | 5,0 g |
| 5-bromo-4-chloro-3-indolyl-α-D-glucopyranoside | 0,15 g |
| Sodium desoxycholate | 0,25 g |
| Ammonium iron(III) citrate | 1,0 g |
| Sodium thiosulfate | 1,0 g |
pH of the ready-to-use medium at 25°C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ Cronobacter | 01043 | 10 plates/box or as per customer request. |
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