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BacterChrom™ Pasteurella Plates
- Ready-to-use chromogenic medium on 90mm plates for the detection of Pasteurellaceae
- Code: 01035
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ Pasteurella is a selective and chromogenic medium designed for the detection, isolation, and differentiation of Pasteurellaceae bacteria, including Histophilus somni, Pasteurella multocida, and Mannheimia haemolytica. These bacteria are commonly associated with bovine respiratory disease complex and can cause significant economic losses in livestock production due to reduced growth rates, milk production declines, and animal deaths.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ Pasteurella uses chromogenic substrates that react with specific enzymatic activities of target bacteria, producing distinct colony colors for easy differentiation. This medium enhances the rapid detection of pathogenic Pasteurellaceae while suppressing background flora, providing high specificity and facilitating the identification of bacterial infections.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Direct streaking sample onto the plate
- Incubate in aerobic conditions at 37°C for 18 – 24 hours
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| Pasteurellaceae | pink to mauve |
| Coliforms | inhibited or blue to steel blue |
| Other Gram (-) bacteria | inhibited or colorless to light pink |
| Yeast and Gram (+) bacteria | inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC and NCTC reference strains.
| Reference strains | Incubation conditions | Expected results |
| P. multocida ATCC 43137 | Incubate for 24 hours at 35 – 37 °C | Mauve colonies |
| M. haemolytica ATCC 33396 | Mauve colonies | |
| E. coli ATCC 35218 | Grey-blue colonies | |
| E. faecalis ATCC 29212 | No growth |
For 1 liter of medium
| Peptone | 25,0 g |
| Chromogenic mix and Selective mix | 0,2 g |
| Salt | 5,0 g |
| Agar | 15,0 g |
| Selective mix (supplement) | 1,0 g |
| Growth factor (supplement) | 150 mL |
pH of the ready-to-use medium at 25°C: 7,0 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ Pasteurella | 01035 | 10 plates/box or as per customer request. |
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