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BacterPlate™ Blood Agar
- Ready-to-use medium on 90mm plates for culturing and differentiating hemolytic phenotypes α, β, and ɤ.
- Code: 05004
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Blood Agar is a versatile culture medium widely utilized for the cultivation and isolation of a broad range of microorganisms. It plays a critical role in in vitro diagnostics and microbiological quality control testing. Furthermore, it enables the differentiation of bacterial species based on hemolytic reactions (alpha, beta, and gamma hemolysis).
- The typical composition of base medium (Columbia Agar) corresponds to that defined in European (EP), United States (UP) and Japanese (JP) Pharmacopeias.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLE
- Peptones included in the composition of the medium favor the excellent growth of colonies.
- Yeast extract is a source of vitamin B complex.
- Starch is a detoxifying agent and also an energy source.
- Defibrinated sheep blood provides X factor (heme), essential for the growth of many fastidious bacteria, and allows the detection of hemolytic reactions.
- Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
- Inoculate in order to obtain isolated colonies.
- Incubate at 37 °C for 24 to 48 hours in optimal conditions for the culture of the inoculated germs.
RESULTS
Observe the bacterial growth.
- Beta hemolysis
- Streptococci belonging to Lancefield group A appear as small, grey colonies, translucid or opaque, surrounded by a zone of beta hemolysis. Other bacteria may present the same type of hemolysis: Listeria, hemolytic Staphylococci, Escherichia coli and Pseudomonas.
- Staphylococci appear as opaque, yellow-gold or white colonies, with or without type β hemolysis zones.
- Listeria present small zones of beta hemolysis.
- Bacillus cereus form a clear zone surrounding the colonies.
- Alpha hemolysis
- Pneumococci appear as flat, shiny, grey and occasionally mucoid colonies surrounded by a zone of narrow, greenish hemolysis referred to as alpha hemolysis.
- CAMP Factor
- Group B Streptococci produce an extracellular, thermoresistant substance (CAMP Factor) which provokes a triangle of total hemolysis in a zone of incomplete staphylococcal hemolysis, at the junction of the two cultures.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation Conditions | Standard (PR) |
| S. pneumonia ATCC 49619 | Incubate for 24 – 48 hours, at 35 – 37°C, with inoculum ≤ 103 – 104 CFU/ mL | Good growth, α hemolysis. |
| S. pyogenes ATCC 19615 | Good growth, hemolysis. | |
| E. faecalis ATCC 29212 | Good growth, ɤ hemolysis. |
For 1 liter of medium
| Polypeptone | 23,0 g |
| Starch | 1,0 g |
| Sodium chloride | 5,0 g |
| Defibrinated sheep blood | 50 – 70 mL |
| Agar | 13,5 g |
pH of the ready-to-use medium at 250C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ BacterPlate™ Blood Agar | 05004 | 10 plates/ box or as per customer request. |
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