Vn
BacterPlate™ Blood Agar
- Ready-to-use medium on 90mm plates for culturing and differentiating hemolytic phenotypes α, β, and ɤ.
- Code: 05004
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Blood Agar is a versatile culture medium widely utilized for the cultivation and isolation of a broad range of microorganisms. It plays a critical role in in vitro diagnostics and microbiological quality control testing. Furthermore, it enables the differentiation of bacterial species based on hemolytic reactions (alpha, beta, and gamma hemolysis).
- The typical composition of base medium (Columbia Agar) corresponds to that defined in European (EP), United States (UP) and Japanese (JP) Pharmacopeias.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLE
- Peptones included in the composition of the medium favor the excellent growth of colonies.
- Yeast extract is a source of vitamin B complex.
- Starch is a detoxifying agent and also an energy source.
- Defibrinated sheep blood provides X factor (heme), essential for the growth of many fastidious bacteria, and allows the detection of hemolytic reactions.
- Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
- Inoculate in order to obtain isolated colonies.
- Incubate at 37 °C for 24 to 48 hours in optimal conditions for the culture of the inoculated germs.
RESULTS
Observe the bacterial growth.
- Beta hemolysis
- Streptococci belonging to Lancefield group A appear as small, grey colonies, translucid or opaque, surrounded by a zone of beta hemolysis. Other bacteria may present the same type of hemolysis: Listeria, hemolytic Staphylococci, Escherichia coli and Pseudomonas.
- Staphylococci appear as opaque, yellow-gold or white colonies, with or without type β hemolysis zones.
- Listeria present small zones of beta hemolysis.
- Bacillus cereus form a clear zone surrounding the colonies.
- Alpha hemolysis
- Pneumococci appear as flat, shiny, grey and occasionally mucoid colonies surrounded by a zone of narrow, greenish hemolysis referred to as alpha hemolysis.
- CAMP Factor
- Group B Streptococci produce an extracellular, thermoresistant substance (CAMP Factor) which provokes a triangle of total hemolysis in a zone of incomplete staphylococcal hemolysis, at the junction of the two cultures.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation Conditions | Standard (PR) |
| S. pneumonia ATCC 49619 | Incubate for 24 – 48 hours, at 35 – 37°C, with inoculum ≤ 103 – 104 CFU/ mL | Good growth, α hemolysis. |
| S. pyogenes ATCC 19615 | Good growth, hemolysis. | |
| E. faecalis ATCC 29212 | Good growth, ɤ hemolysis. |
For 1 liter of medium
| Polypeptone | 23,0 g |
| Starch | 1,0 g |
| Sodium chloride | 5,0 g |
| Defibrinated sheep blood | 50 – 70 mL |
| Agar | 13,5 g |
pH of the ready-to-use medium at 250C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ BacterPlate™ Blood Agar | 05004 | 10 plates/ box or as per customer request. |
Related products
PassBox With Filters – Tecra-PB.HEPA.BioSafe
- Structure: Double-layer design with an exterior made of 1.2mm thick electro-galvanized steel and interior made of SUS 304/316 stainless steel.
- Centrifugal fan motor: EBMpapst – Germany, energy-efficient.
- Polycarbonate viewing windows.
- Fitted with an electric interlock system.
- Warranty: 3 years.
BacterChrom™ AOLA Plates
- Ready-to-use chromogenic medium on 90mm plates for the detection, enumeration and isolation of Listeria monocytogenes and Listeria spp.
- Code: 01046
- Packaging: 10 plates/box or as per customer requirem ents.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterChrom™ Mastitis Plates
- Ready-to-use chromogenic medium on 90mm plates for the isolation and differentiation of the main pathogens involved in Mastitis infections
- Code: 01039
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
Class I Biological Safety Cabinet, HUYAir Bio1
Class I Biological Safety Cabinet, HUYAir Bio1 is designed to provide safety protection for operators and the environment during direct handling of contaminated samples with a high potential for disease transmission. The Class 1 cabinet is a cost-effective alternative to Class 2 biological safety cabinets, suitable for applications that do not require product protection.
- Standard compliance: NSF 49, EN 12469.
- The cabinet meets the Class 1 Biological Safety Cabinet standards issued by the National Institute of Hygiene and Epidemiology.
- Size standard: 900mm | 1200mm | 1500mm | 1800mm.
- Warranty: 3 years.
BacterPlate™ Mueller Hinton Agar, Glucose-Methylene Blue
- Ready-to-use medium on 90mm plates for testing susceptibility with antifungal disc diffusion of yeasts.
- Code: 05033
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterTube™ BHI Ge/Cl
- A selective enrichment medium for Streptococcus and fungi
- Code: 08015
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
Vertical Laminar Flow Cabinet, HUYAir VLF
The HUYAir VLF is a series of highly efficient Vertical Laminar Flow (VLF) Microbiological Safety Cabinets designed to protect samples, equipment, and other components in the work area from molecular to highly sensitive applications. It is ideal for use with non-hazardous and easily accessible materials in the work area as desired. The key feature of the VLF cabinets is to create a clean working environment for a wide range of applications and utilize UV lamps for sterilization, cleaning the work area, and internal components during operation with the LCD digital control system.
- Complies with standards: NSF 49, EN 12469.
- The cabinet is certified by the National Institute of Hygiene and Epidemiology.
- Size standard: 900mm | 1200mm | 1500mm | 1800mm
- Warranty: 03 years.
BacterChrom™ Streptococcus Plates
- Ready-to-use chromogenic medium on 90mm plates for the isolation and differentiation of Streptococcus spp.
- Code: 01037
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterPlate™ Chocolate Agar with VCN (CA VCN)
- Ready-to-use medium on 90mm plates for the selective culture of Neisseria gonorrhoeae and Neisseria meningitidis.
- Code: 05010
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
Powder Weighing Balance Enclosures, HUYAir PWB
HUYlab’s Powder Weighing Balance Enclosures provides protection for users during the weighing, measuring, pouring, mixing, and other related activities involving dust, particles, and powders of hazardous compounds and/or liquid chemicals.
- Complies with standards: NSF 49, EN 12469.
- The cabinet is certified by the National Institute of Hygiene and Epidemiology.
- Size standard: 700mm | 900mm | 1200mm | 1500mm.
- Warranty: 03 years.