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BacterPlate™ Blood Agar with Nalidixic Acid (BANg)
- Ready-to-use medium on 90mm plates for the selective culture of Staphylococcus and Streptococcus. Differentiation of α, β, and ɤ hemolytic phenotypes.
- Code: 05005
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Blood Agar with Nalidixic Acid (BANg) is a ready-to-use selective medium designed for the isolation and differentiation of Staphylococcus and Streptococcus species. It facilitates the identification of hemolytic phenotypes (α, β, and ɣ hemolysis) and provides targeted cultivation for diagnostic applications. This medium is ideal for clinical microbiology laboratories to support the detection of pathogenic Gram-positive cocci while suppressing non-target organisms..
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- The BANg medium is composed of:
- Nalidixic Acid: Inhibits the growth of Gram-negative bacteria, enhancing selectivity for Gram-positive species.
- Defibrinated Blood: Provides heme (X factor) essential for bacterial growth and hemolytic activity differentiation.
- Enriched Nutrients: Supports robust growth of Staphylococcus and Streptococcus
- This selective formulation enables clear visualization and differentiation of α, β, and ɣ hemolytic reactions, streamlining diagnostic workflows.
- Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
- Inoculate in order to obtain isolated colonies.
- Incubate at 37°C for 18 – 24 hours in optimal conditions for the culture of the inoculated germs.
RESULTS
Observe the bacterial growth.
- Beta hemolysis
- Streptococci belonging to Lancefield group A appear as small, grey colonies, translucid or opaque, surrounded by a zone of beta hemolysis. Other bacteria may present the same type of hemolysis: Listeria, hemolytic Staphylococci, Escherichia coli and Pseudomonas.
- Staphylococci appear as opaque, yellow-gold or white colonies, with or without type β hemolysis zones.
- Listeria present small zones of beta hemolysis.
- Bacillus cereus form a clear zone surrounding the colonies.
- Alpha hemolysis
- Pneumococci appear as flat, shiny, grey and occasionally mucoid colonies surrounded by a zone of narrow, greenish hemolysis referred to as alpha hemolysis.
- CAMP Factor
- Group B Streptococci produce an extracellular, thermoresistant substance (CAMP Factor) which provokes a triangle of total hemolysis in a zone of incomplete staphylococcal hemolysis, at the junction of the two cultures.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation Conditions | Standard (PR) |
| S. pneumonia ATCC 49619 | Incubate for 18 – 24 hours, at 35 – 37°C | Good growth, α hemolysis. |
| S. pyogenes ATCC 19615 | Good growth, hemolysis. | |
| S. aureus ATCC 25923 | Good growth, ɤ hemolysis. | |
| E. coli ATCC 35218 | Inhibited |
For 1 liter of medium
| Polypeptone | 23,0 g |
| Starch | 1,0 g |
| Sodium chloride | 5,0 g |
| Defibrinated sheep blood | 50 – 70 mL |
| Nalidixic acid | 0,04 g |
| Agar | 13,5 g |
pH of the ready-to-use medium at 250C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ Blood Agar with Nalidixic Acid (BANg) | 05005 | 10 plates/ box or as per customer request. |
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