Vn
BacterPlate™ Blood Agar with Nalidixic Acid (BANg)
- Ready-to-use medium on 90mm plates for the selective culture of Staphylococcus and Streptococcus. Differentiation of α, β, and ɤ hemolytic phenotypes.
- Code: 05005
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Blood Agar with Nalidixic Acid (BANg) is a ready-to-use selective medium designed for the isolation and differentiation of Staphylococcus and Streptococcus species. It facilitates the identification of hemolytic phenotypes (α, β, and ɣ hemolysis) and provides targeted cultivation for diagnostic applications. This medium is ideal for clinical microbiology laboratories to support the detection of pathogenic Gram-positive cocci while suppressing non-target organisms..
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- The BANg medium is composed of:
- Nalidixic Acid: Inhibits the growth of Gram-negative bacteria, enhancing selectivity for Gram-positive species.
- Defibrinated Blood: Provides heme (X factor) essential for bacterial growth and hemolytic activity differentiation.
- Enriched Nutrients: Supports robust growth of Staphylococcus and Streptococcus
- This selective formulation enables clear visualization and differentiation of α, β, and ɣ hemolytic reactions, streamlining diagnostic workflows.
- Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
- Inoculate in order to obtain isolated colonies.
- Incubate at 37°C for 18 – 24 hours in optimal conditions for the culture of the inoculated germs.
RESULTS
Observe the bacterial growth.
- Beta hemolysis
- Streptococci belonging to Lancefield group A appear as small, grey colonies, translucid or opaque, surrounded by a zone of beta hemolysis. Other bacteria may present the same type of hemolysis: Listeria, hemolytic Staphylococci, Escherichia coli and Pseudomonas.
- Staphylococci appear as opaque, yellow-gold or white colonies, with or without type β hemolysis zones.
- Listeria present small zones of beta hemolysis.
- Bacillus cereus form a clear zone surrounding the colonies.
- Alpha hemolysis
- Pneumococci appear as flat, shiny, grey and occasionally mucoid colonies surrounded by a zone of narrow, greenish hemolysis referred to as alpha hemolysis.
- CAMP Factor
- Group B Streptococci produce an extracellular, thermoresistant substance (CAMP Factor) which provokes a triangle of total hemolysis in a zone of incomplete staphylococcal hemolysis, at the junction of the two cultures.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation Conditions | Standard (PR) |
| S. pneumonia ATCC 49619 | Incubate for 18 – 24 hours, at 35 – 37°C | Good growth, α hemolysis. |
| S. pyogenes ATCC 19615 | Good growth, hemolysis. | |
| S. aureus ATCC 25923 | Good growth, ɤ hemolysis. | |
| E. coli ATCC 35218 | Inhibited |
For 1 liter of medium
| Polypeptone | 23,0 g |
| Starch | 1,0 g |
| Sodium chloride | 5,0 g |
| Defibrinated sheep blood | 50 – 70 mL |
| Nalidixic acid | 0,04 g |
| Agar | 13,5 g |
pH of the ready-to-use medium at 250C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ Blood Agar with Nalidixic Acid (BANg) | 05005 | 10 plates/ box or as per customer request. |
Related products
BacterPlate™ Chocolate Agar with Neomycin
- Ready – to – use medium for culturing fastidious microorganisms, particularly Neisseria and Haemophilus.
- Code: 05016
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterPlate™ Meat Extract Agar
- Ready-to-use medium on 90mm plates for the cultivation of non-fastidious microorganisms
- Code: 05025
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterPlate™ Chocolate Agar with Multivitox
- Medium for culturing fastidious microorganisms, particularly Neisseria and Haemophilus.
- Code: 05017
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterPlate™ Dnase Agar
- Ready-to-use medium on 90mm plates for detecting the deoxyribonuclease enzyme of microorganisms, particularly Staphylococci.
- Code: 05030
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
Horizontal Laminar Flow Cabinet, HUYAir HLF
The Horizontal Laminar Flow Cabinet, HUYAir HLF designed to be used with non-hazardous and easily accessible materials within the working area. The distinctive feature of this HLF cabinet is to create a clean working environment within a specified range, widely applicable for cleaning the working area and internal components of the cabinet during operations, with a digital LCD control system.
- Complies with standards: NSF 49, EN 12469.
- The cabinet is certified by the National Institute of Hygiene and Epidemiology.
- Size standard: 900mm | 1200mm | 1500mm | 1800mm.
- Warranty: 03 years.
BacterChrom™ AOLA Plates
- Ready-to-use chromogenic medium on 90mm plates for the detection, enumeration and isolation of Listeria monocytogenes and Listeria spp.
- Code: 01046
- Packaging: 10 plates/box or as per customer requirem ents.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterChrom™ Pasteurella Plates
- Ready-to-use chromogenic medium on 90mm plates for the detection of Pasteurellaceae
- Code: 01035
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
Powder Weighing Balance Enclosures, HUYAir PWB
HUYlab’s Powder Weighing Balance Enclosures provides protection for users during the weighing, measuring, pouring, mixing, and other related activities involving dust, particles, and powders of hazardous compounds and/or liquid chemicals.
- Complies with standards: NSF 49, EN 12469.
- The cabinet is certified by the National Institute of Hygiene and Epidemiology.
- Size standard: 700mm | 900mm | 1200mm | 1500mm.
- Warranty: 03 years.
BacterPlate™ Blood Agar with Gentamycin (BAGe)
- Ready-to-use medium on 90mm plates for the selective culture of Streptococcus. Differentiation of α, β, and ɤ hemolytic phenotypes.
- Code: 05006
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
Ducted Fume Hood, HUYAir FH
The Ducted Fume Hood, HUYAir FH used to control and treat pollutant emissions in industrial and chemical applications, particularly when working with strong acids and corrosive chemicals, to protect the health of workers.
- The quality of the Toxic Fume Hood, HUYAir FH, meets the ASHRAE 110 standard.
- Size standard: 1200mm | 1500mm | 1800mm.
- Warranty: 3 years.