BacterPlate™ Blood Agar with Nalidixic Acid (BANg)

Ready-to-use medium on 90mm plates for the selective culture of Staphylococcus and Streptococcus. Differentiation of α, β, and ɤ hemolytic phenotypes.
Code: 05005
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterPlate™ Blood Agar with Nalidixic Acid (BANg) is a ready-to-use selective medium designed for the isolation and differentiation of Staphylococcus and Streptococcus species. It facilitates the identification of hemolytic phenotypes (α, β, and ɣ hemolysis) and provides targeted cultivation for diagnostic applications. This medium is ideal for clinical microbiology laboratories to support the detection of pathogenic Gram-positive cocci while suppressing non-target organisms..
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

The BANg medium is composed of:
- Nalidixic Acid: Inhibits the growth of Gram-negative bacteria, enhancing selectivity for Gram-positive species.
- Defibrinated Blood: Provides heme (X factor) essential for bacterial growth and hemolytic activity differentiation.
- Enriched Nutrients: Supports robust growth of Staphylococcus and Streptococcus
This selective formulation enables clear visualization and differentiation of α, β, and ɣ hemolytic reactions, streamlining diagnostic workflows.

Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
Inoculate in order to obtain isolated colonies.
Incubate at 37°C for 18 – 24 hours in optimal conditions for the culture of the inoculated germs.

RESULTS

Observe the bacterial growth.

Beta hemolysis
- Streptococci belonging to Lancefield group A appear as small, grey colonies, translucid or opaque, surrounded by a zone of beta hemolysis. Other bacteria may present the same type of hemolysis: Listeria, hemolytic Staphylococci, Escherichia coli and Pseudomonas.
- Staphylococci appear as opaque, yellow-gold or white colonies, with or without type β hemolysis zones.
- Listeria present small zones of beta hemolysis.
- Bacillus cereus form a clear zone surrounding the colonies.
Alpha hemolysis
- Pneumococci appear as flat, shiny, grey and occasionally mucoid colonies surrounded by a zone of narrow, greenish hemolysis referred to as alpha hemolysis.
CAMP Factor
- Group B Streptococci produce an extracellular, thermoresistant substance (CAMP Factor) which provokes a triangle of total hemolysis in a zone of incomplete staphylococcal hemolysis, at the junction of the two cultures.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.