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BacterBag™ Maximum Recovery Diluent
- Protective and isotonic medium used for maximal recovery of microorganisms from a variety of sources.
- Code: 10004
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
- INTENDED USE
BacterBag™ Maximum Recovery Diluent is a protective and isotonic medium used for maximal recovery of microorganisms from a variety of sources.
- PRINCIPLE
BacterBag™ Maximum Recovery Diluent is formulated as recommended by ISO Committee for use as an isotonic diluent. Standard methods for the examination of foodstuffs require sample dilution to be carried out accurately to estimate the number of microorganisms. Maximum Recovery Diluent combines protective effect of Peptone with the osmotic balance of physiological saline. The low concentration of Peptone helps to maintain the organisms for 1 – 2 hours of dilution without multiplication. The isotonic property of the diluent ensures the recovery of organisms from various sources, which may be vulnerable in distilled water or aqueous suspensions.
Preparation of stock solution
- Add Xg or XmL of product for analysis to BacterBag™ Maximum Recovery Diluent medium to make a 1:10 dilution.
- Mix well.
Preparation of serial dilutions
- Add 1 mL of stock suspension to a tube containing 9 mL of BacterBag™ Maximum Recovery Diluent.
- Mix well.
- Repeat the operation until the desired dilution concentration is reached.
BacterLab ensures the quality of each product batch of the medium by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Escherichia coli ATCC 25922 (WDCM 00013) | 35 – 37°C for 18 – 24 hours | Good growth |
| Staphylococcus aureus ATCC 25923 (WDCM 00034) | Good growth |
For 1L of medium (reference):
| Peptone | 1,0 g |
| Sodium chloride | 8,5 g |
pH of the ready-to-use medium at 25°C: 7,0 ± 0,2
| Product Name | Code | Packaging |
| BacterBag™ Maximum Recovery Diluent | 10001 | 5 liter/ bag or as per customer requirements. |
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