BacterBag™ Dry Buffered Peptone Water (BPW)

Dry powder form of Buffered Peptone Water used for pre-enrichment in the Salmonella testing process
Code: 10013
Packaging: Equivalent to 20L of medium per bag or as per customer requirements.
Storage Conditions: 8 – 25°C.
Transportation: Ambient temperature.
Shelf Life: 36 months from the date of manufacture.

INTENDED USE

BacterBag™ Dry Buffered Peptone Water is the dry form of Buffered Peptone Water, used in the pre-enrichment step of microbiological testing. This medium is used to enrich a variety of bacteria, including those that have undergone sub-lethal treatments such as spray drying, pasteurization, and other preservation methods. It helps recover and enrich bacteria such as Salmonella and Listeria monocytogenes before performing further selective tests.

PRINCIPLES

Buffered Peptone Water is a non-selective medium used to enrich bacteria. The peptones in the medium provide essential carbon, nitrogen, and vitamins for bacterial growth. The buffering system, consisting of disodium phosphate and monopotassium phosphate, helps maintain a stable pH and prevents damage to bacteria during recovery.
High-quality peptones in the medium help bacteria recover and grow well, promoting the growth and recovery of stressed bacteria.

Add the appropriate amount of sterile distilled water into the BacterBag™ Dry Buffered Peptone Wate packet according to the ratio provided on the packaging to create a complete solution.
Homogenize until the medium is completely dissolved.

1. Direct Culturing from the Original Sample

Take 10 or 25 g of the sample to be analyzed and add it to a volumetric flask. Then, add 90 or 225 mL of the BacterBag™ Dry Buffered Peptone Water that has been dissolved in water.
Mix thoroughly using an appropriate homogenizer.
For enriching bacteria such as Salmonella or Enterobacteria, the sample should be incubated following the appropriate analytical procedure to ensure accurate results.

2. Preparing Serial Dilution Solutions

Take 1 mL of the stock suspension and add it to a test tube containing 9 mL of the BacterBag™ Dry Buffered Peptone Water that has been dissolved in water.
Mix the solution thoroughly.
Continue diluting as required by repeating the steps above to achieve the desired dilution

RESULTS

This medium is used to recover and enrich bacteria in samples. After incubation, secondary culturing should be performed in other selective media to identify the bacteria.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

For 1 liter of medium

Peptone	10,0 g
Sodium Choloride	5,0 g
Disodium phosphate, anhydrous	3,57 g
Monopotassium phosphate, anhydrous	1,5 g

pH of the ready-to-use medium at 25°C: 7,0 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterBag™ Dry Buffered Peptone Water (BPW)	10013	Equivalent to 20L of medium per bag or as per customer requirements.