BacterChrom™ Bacillus Plus Agar Plates

Ready-to-use medium on 90mm plates for detecting and quantifying Bacillus cereus.
Code: 01049
Packaging: 10 plates/box or as per customer requirem ents.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterChrom^TM Bacillus Plus Agar is used for the detection and enumeration of spores and vegetative cells of Bacillus cereus in food products. It is used in the enumeration at 30°C of presumptive Bacillus cereus according to the standard (NF EN ISO 7932). It is also recommended in the detection or for small numbers method NF EN ISO 21871.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

Tryptone and meat extract favor the growth of Bacillus cereus.
Sterile egg yolk emulsion is used to detect the presence of lecithinase present in most Bacillus cereus strains.
Insoluble breakdown products of egg yolk lecithin accumulate around the colonies, forming a whitish precipitate.
Mannitol is used to differentiate contaminating microorganisms which ferment it, causing phenol red to turn yellow.
Polymyxin is used to inhibit accompanying microflora when the tested sample is heavily contaminated.

Enumeration of Bacillus cereus at 30 °C (NF EN ISO 7932)
- Dry the plates in an incubator with the covers partially removed.
- Transfer 0,1 mL of the sample to analyze and its serial dilutions to the BacterChrom^TM Bacillus Plus Agar
- Spread the inoculum on the surface of the agar with a sterile triangle.
- Incubate at 30°C for 18 to 24 hours. If the colonies are not readily visible, prolong the incubation an additional 24 hours
Detection or low bacterial numbers of Bacillus cereus (NF EN ISO 21871)
- On the surface of the BacterChrom^TM Bacillus Plus Agar, re-inoculate a loop of each enrichment broth.
- Incubate at 30°C for 18 to 24 hours. If the colonies are not readily visible, prolong the incubation an additional 24 hours

RESULTS

Presumed colonies of Bacillus cereus are pink (mannitol-negative) and almost always surrounded by a halo of precipitate, indicating the production of lecithinase. In general they measure 2 to 5 mm and present irregular, stringy borders

NOTE:

If the plates have a high level of contaminating flora that ferment mannitol, the pink color of the agar and the Bacillus cereus colonies may cause visibility problems in identification.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

For 1 liter of medium

pH of the ready-to-use medium at 25°C: 7,0 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterChrom™ Bacillus Plus Agar	01049	10 plates/box or as per customer request.