BacterChrom™ COL_R Agar Plates
- Ready-to-use chromogenic medium on 90mm plates for qualitative analysis of colistin-resistant gram-negative bacteria (COL-R).
- Code: 01016
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ COL_R Agar is a selective and differential chromogenic culture medium, intended for use in the qualitative direct detection of gastrointestinal colonization with colistin-resistant Gram-negative bacteria (COL-R) to aid in the prevention and control of COL-R in healthcare settings.Additionally, the medium can act as an early warning indicator in diagnostic testing, highlighting the possible presence of COL-R bacteria. It can also be used in food, animal feed, livestock, and environmental sample analyses.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ COL_R Agar utilizes chromogenic substrates and selective agents to differentiate colistin-resistant Gram-negative bacteria. The chromogenic components react with specific enzymatic activities, producing distinct colony appearances for easy presumptive identification. Selective agents suppress non-target organisms, ensuring specificity and enhancing the accuracy of detection.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Direct streaking sample onto the plate
- Incubate in aerobic conditions at 35 – 37°C for 18 – 24 hours
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| COL-R E. coli | dark pink to reddish |
| COL-R Coliforms | metallic blue |
| COL-R Pseudomonas | translucent, +/- natural pigmentation cream to green |
| COL-R Acinetobacter | cream, opaque |
| Other COL-R Gram (-) | colourless, natural pigmentation |
| COL-S Gram (-) bacteria | inhibited |
| Gram (+) bacteria, yeasts | inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| MCR-1 E. coli NCTC 13846 | Incubate for 18 – 24 hours at 35 – 37 °C | Dark pink to red colonies |
| COL-S E. coli ATCC 35218 | Mostly inhibited | |
| S. marcescens ATCC 13880 | Blue colonies | |
| E. faecalis ATCC 29212 | Inhibited |
For 1 liter of medium
| Agar | 15,0 g |
| Peptones | 20,0 g |
| Salt | 5,0 g |
| Chromogenic and selective mix | 0,8 g |
| Growth factors | 1,7 g |
| Supplement (S) | 2mL |
pH of the ready-to-use medium at 25°C: 7,1 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ COL_R Agar | 01016 | 10 plates/box or as per customer request. |
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