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BacterChrom™ Mastitis Plates
- Ready-to-use chromogenic medium on 90mm plates for the isolation and differentiation of the main pathogens involved in Mastitis infections
- Code: 01039
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ Mastitis is a selective chromogenic medium designed for the isolation and differentiation of the main Gram (+) pathogens involved in Mastitis infections.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ Mastitis uses chromogenic substrates that react with specific enzymatic activities of target bacteria, leading to distinct colony colors for differentiation. The medium incorporates selective agents to inhibit the growth of non-target organisms, ensuring high specificity and simplifying the identification process. This design supports the rapid and accurate detection of Gram-positive bacteria associated with mastitis.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Direct streaking sample onto the plate
- Incubate in aerobic conditions at 35 – 37°C for 24 hours.
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| S. agalactiae | turquoise blue |
| S. uberis | metallic blue |
| S. aureus | pink |
| Gram (-) bacteria | inhibited |
| Other microorganism | various |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| S. agalactiae ATCC 12386 | Incubate for 24 hours at 35 – 37 °C | Turquoise blue colonies |
| S. aureus ATCC 25923 | Pink colonies | |
| E. coli ATCC 35218 | Inhibited | |
| C. albicans ATCC 10231 | Inhibited |
For 1 liter of medium
| Peptone and yeast | 20,0 g |
| Chromogenic mix | 2,4 g |
| Salt | 5,0 g |
| Agar | 15,0 g |
| Growth factor (supplement) | 8 mL |
pH of the ready-to-use medium at 25°C: 6,9 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ Mastitis | 01039 | 10 plates/box or as per customer request. |
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