BacterChrom™ MH Orientation Agar Plates

Ready-to-use chromogenic medium on 90mm plates for differentiating and testing antibiotic susceptibility (antibiotic susceptibility testing) on the same plate.
Code: 01020
Packaging: 10 plates/box or as per customer requirements.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterChrom™ MH OrientationAgar is a chromogenic Mueller Hinton medium designed for dual purposes: routine detection of common urinary tract infections (UTIs) and rapid antimicrobial susceptibility testing. The medium is particularly suitable for critical cases such as ventilator-associated pneumonia (VAP) in ICU patients, where timely results can significantly impact patient outcomes by aiding early therapeutic decisions.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterChrom™ MH Orientation Agar combines the properties of chromogenic and Mueller Hinton media, offering dual functionalities for microbial growth and differentiation. Chromogenic substrates in the medium enable specific enzymes to produce characteristic colony colors, facilitating easy identification. The selective components ensure high specificity by suppressing non-target organisms, supporting both identification and antimicrobial susceptibility testing.

Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
Spread a bacterial suspension according to classical procedure. Alternatively perform a direct spreading of the sample on the plate allowing a confluent growth
Incubate in aerobic conditions at 35 – 37°C for 18 – 24 hours (in some cases a first reading can be done as soon as 8 hours after incubation).

RESULTS

Microorganism	Typical colony apprearance
Gram (-)
E. coli	Dark pink to reddish
Klebsiella, Enterobacter, Citrobacter, Serratia	Metallic blue (+/- reddish halo)
Proteus, Morganella, Providencia	Brown halo
Proteus vulgaris	Blue with brown halo
Pseudomonas	Translucent (+/- natural pigmentation cream to green)
Acinetobacter	Cream
Stenotrophomonas	Colourless
Gram (+)
Enterococcus	Turquoise blue
S. aureus	Golden, opaque, small
S. saprophyticus	Pink, opaque, small
Streptococcus Group B	Light blue

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.