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INTENDED USE
- BacterPlate™ Cetrimide Agar is a selective medium for the isolation and enumeration of Pseudomonas aeruginosa in biological samples of animal origin and in pharmaceutical and cosmetic products.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- Cetrimide (cetyltrimethylammonium bromide) is a quaternary ammonium compound which inhibits a large number of bacteria including species of Pseudomonas other than Pseudomonas aeruginosa.
- The production of pyocyanin (a blue, non-fluorescent pigment soluble in water and chloroform) is stimulated by magnesium chloride and potassium sulfate.
- The medium also favors the production of fluorescent pigments (pyoverdins) by some strains of Pseudomonas aeruginosa.
- Most Pseudomonas aeruginosa can be identified by the characteristic grapelike odor of aminoacetophenone.
- Dry the plates in an incubator with the covers partially removed.
- Inoculate by streaking with the aid of a sterile loop onto the surface of the agar.
- Incubate at 30 – 35°C:
- for 24 to 48 hours, as defined in the Directive NF EN ISO 22717 for cosmetic products.
- for 18 to 72 hours following the Pharmacopoeia, in the case of pharmaceutical products.
RESULTS
Pseudomonas aeruginosa can present the following aspects:
- Characteristic yellow-green pigmentation and a fluorescence under UV light at 254 nm.
- Grayish, mucoid colonies, with or without pigmentation.
NOTE: Occasionally, strains of Klebsiella, Enterobacter, Citrobacter, Proteus, Providencia, Alcaligenes and Aeromonas may also grow, causing a slight yellowing of the medium. This color is easily differentiated from fluorescein production since the former does not fluoresce.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| S. aureus ATCC 25923 | 35 – 37°C after 24 – 48 hours | Inhibited |
| E. coli ATCC 35218 | Inhibited | |
| P. aeruginosa ATCC 9027 | Good growth |
For 1 liter of medium
| Pancreatic digest of gelatin | 20,0 g |
| Glycerol | 10 mL |
| Cetrimide | 0,3 g |
| Magnesium chloride | 1,4 g |
| Sulfate de potassium | 10,0 g |
| Agar | 13,6 g |
pH of the ready-to-use medium at 25°C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ Cetrimide Agar | 05031 | 10 plates/ box or as per customer request. |
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