BacterPlate™ Mannitol Salt Agar (MSA)
- Ready-to-use medium on 90mm plates for the selective culture of Staphylococcus, differentiating between Staphylococcus aureus and Staphylococcus epidermidis.
- Code: 05021
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Mannitol Salt Agar (MSA) is used for the selective isolation, detection and enumeration of pathogenic staphylococci in filterable water as in swimming pools, potable water or spas. It is also used for the detection of Staphylococcus aureus according to the Pharmacopeia and in cosmetic products.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- The high sodium chloride concentration inhibits the growth of most bacteria other than staphylococci.
- Mannitol fermentation, shown by the color change of the pH indicator (phenol red) to yellow orients the diagnosis.
- The demonstration of pathogenic staphylococci is generally confirmed by a coagulase test.
1. Detection of Staphylococcus aureus (Pharmacopeia, NF EN ISO 22718)
- Dry in an incubator the plates with the covers partially removed.
- Transfer 0,1 mL of the product to analyze to the surface of the plates.
- Spread the inoculum on the surface of the medium with a sterile triangle.
- Incubate at 30 – 35 °C for 18 to 24 hours.
2. Water testing by membrane filtration
- Aseptically filter the appropriate volume of water through a nitrocellulose membrane.
- Place the membrane, filtered side up, on the surface of the plates, insuring a complete contact.
- Incubate at (36 ± 2)°C for (44 ± 4) hours.
RESULTS
- Count the characteristic colonies.
- Pathogenic staphylococci form luxuriant pigmented colonies, surrounded by a yellow ring due to the fermentation of mannitol.
- Non-pathogenic staphylococci generally give rise to small red colonies which do not change the color of the medium.
- Several strains of Staphylococcus epidermidis can ferment mannitol.
- After 48 hours of incubation, several strains of enterococci, Bacillus, Micrococcus and Serratia may grow
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
Reference Strains | Incubation Conditions | Expected results |
S. aureus ATCC 25923 | 30 – 35°C for
18 – 24 hours |
Good growth, yellow colonies |
S. epidermidis ATCC 12228 | Good growth, pink colonies | |
E. coli ATCC 35218 | No growth |
For 1 liter of medium
Lab-Lemco’ powder | 1,0 g |
Peptone | 10,0 g |
Mannitol | 10,0 g |
Sodium chloride | 75,0 g |
Phenol red | 0,025 g |
Agar | 15,0 g |
pH of the ready-to-use medium at 25°C: 7,4 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
Product Name | Code | Packaging |
BacterPlate™ Mannitol Salt Agar (MSA) | 05021 | 10 plates/ box or as per customer request. |
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