BacterPlate™ Sabouraud Dextrose Agar (SDA)

Ready-to-use medium on 90mm plates for culturing, differentiating, and quantifying yeasts and molds.
Code: 05027
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 04 months from the date of manufacture.

INTENDED USE

BacterPlate™ Sabouraud Dextrose Agar (SDA) is a classical medium for the culture, isolation and identification of yeasts & molds in sterility tests of pharmaceutical and cosmetic products. It is also used in the specific detection of Candida albicans in pharmaceutical products.
Air sampling of cleanroom control indicators.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

Sabouraud Dextrose Agar is a peptone glucose medium enabling the growth of yeasts and molds.
The acid pH inhibits the majority of secondary flora.

Enumeration of yeasts and molds in non-sterile products, according to the Pharmacopeia

Dry the plates in an incubator with the covers partially removed.
Inoculate with 0,1 mL of the test sample on the surface of the plates and spread around evenly with sterile triangle
Use of membrane filters are also possible; deposit the membrane on the surface of the agar, filtered side up, after filtration of a cosmetic test product.

Cosmetics, demonstration of the efficacy of a neutralizer (NF EN ISO 11930)

Transfer 1 mL of each trial (test sample and controls) into plates.
Pour over roughly 15 mL of molten media maintained at 44 – 47 °C, per plate.
Mix well and let solidify on a cold, flat surface.
Incubate at 30 – 35 °C for 2 to 3 days for the enumeration of Candida albicans.

Note : The media can be used for the enumeration of yeasts and molds in cosmetic products known to be free of contamination by bacteria according to the standard NF EN ISO 16212. In this case, inoculate in depth, on the surface or by filtration and incubate 5 to 7 days at 20 – 25 °C

RESULTS

Following incubation, observe the growth of yeasts and molds.
Enumerate the plates containing less than 100 colonies or thallus.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

PERFORMANCE	STANDARD
Culture conditions: 24 hrs, incubated at 30 – 35°C, inoculum ≤ 10² CFU /mL
C. albicans ATCC 10231	P_R ≥ 70 %
Culture conditions: 48 – 72 hrs, incubated at 20 – 25°C, inoculum ≤ 10³ – 10⁴CFU /mL
S. cerevisiae ATCC 20827	Good growth
A. brasiliensis ATCC 16404	Good growth