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BacterPlate™ Two-Compartment Medium MRSA / VRE
- Ready-to-use medium on 90mm plates for the detection and differentiation of Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin-resistant Enterococcus (VRE).
- Code: 05050
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Two-Compartment Medium MRSA / VRE is a ready-to-use two-compartment agar medium designed for the detection and differentiation of Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin-resistant Enterococcus (VRE). This product is used in microbiological research, clinical diagnostics, and microbiological quality control of food, water, pharmaceuticals, and animal-derived biological samples.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- MRSA Compartment: This is a selective medium containing bile salts, cefoxitin, or oxacillin, which helps isolate Methicillin-resistant Staphylococcus aureus (MRSA). The medium is accompanied by a color indicator that helps identify characteristic colonies (usually green/purple/red depending on the medium composition).
- VRE Compartment: This medium contains selective components such as vancomycin, used to isolate Enterococcus strains resistant to vancomycin (VRE). A color indicator is used to differentiate between VRE (+) and VRE (-) colonies.
- Allow the agar plates to reach room temperature before use.
- Dry the agar plates in an incubator with the lid slightly open.
- Inoculate the sample by spreading it evenly on the surface of the agar using a sterile inoculating loop.
- Incubate at 35–37°C for 18–24 hours.
RESULTS
MRSA Compartment
- MRSA (+): Colonies have a characteristic color (usually pink/purple/red), growing well.
- MRSA (–): No growth or weak growth. (MSSA)
VRE Compartment
- VRE (+): Colonies have a characteristic color, growing well despite the presence of antibiotics.
- VRE (–): No growth or weak growth.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| MRSA Compartment | ||
| S. aureus ATCC 43300 | Incubate at 35–37°C for 18–24 hours. | Good growth, mauve colonies |
| S. aureus ATCC 25923 | Weak growth to no growth. | |
| VRE Compartment | ||
| VRE. faecalis ATCC 51575 | Incubate at 35–37°C for 18–24 hours.
|
Good growth |
| E. faecalis ATCC 29212 | Inhibited, no growth | |
| E. coli ATCC 35218 | Inhibited, no growth | |
| S. aureus ATCC 25923 | Inhibited, no growth | |
For 1 liter of MRSA medium
| Peptone and yeast extract | 40,0 g |
| Sodium chloride | 25,0 g |
| Chromagenic mix | 2,5 g |
| Agar | 15,0 g |
pH of the ready-to-use medium at 25°C: 6,9 ± 0,2
For 1 liter of VRE medium
| Peptone and yeast extract | 20,0 g |
| Sodium chloride | 5,0 g |
| Chromogenic mix | 27,3 g |
| Selective mix | 0,06 g |
| Agar | 15,0 g |
pH of the ready-to-use medium at 25°C: 6,9 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ Two-Compartment Medium MRSA / VRE | 05050 | 10 plates/ box or as per customer request. |
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