Vn
INTENDED USE
- BacterTest™ KOH is one of the two main reagents (along with KOH) in the Voges-Proskauer method, playing an important role in creating the alkaline environment necessary for acetoin (or 3-hydroxybutanone) in the sample to react with α-Naphthol, forming a red or pink complex.
PRINCIPLES
- BacterTest™ KOH is one of the two main reagents in this test, with an important role in the detection of acetoin.
- Acetoin (or 3-hydroxybutanone) is produced during the fermentation of glucose (or other carbohydrates) by some species of bacteria.
- When the culture medium contains acetoin, the α-Naphthol reagent will react with acetoin, forming a red or pink complex.
- KOH is necessary to increase the alkalinity of the medium, helping acetoin to convert into substances that can react with α-Naphthol, creating a recognizable color.
- Bacteria are cultured in prepared MR-VP medium after 24 – 48 hours at 37°C (to allow bacteria time to ferment glucose in the medium).
- Adding 0.5 mL of 40% KOH solution to the sample.
- Then, adding 3 – 4 drops of α-Naphthol reagent to the cultured bacteria sample.
- Reacting within minutes to hours.
- Observing the color change of the medium, reading and recording the results.
RESULTS
- Observe the color change of the environment:
- Red or pink = Positive (Bacteria that produce acetoin)
- Colorless or Yellow = Negative (Bacteria do not produce acetoin).
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| REFERENCE STRAINS | CULTURE CONDITIONS | Α-NAPHTHOL + KOH |
| Culture conditions: 18 – 24 hours, incubated at 35 – 37°C, inoculum ≤ 102 CFU/mL | ||
| K. pneumonia NCTC 13442 | Good growth | (–): Red |
| E. coli ATCC 35218 | Good growth | (+): Yellow or no change |
Refer to the components.
| KOH (kali hydroxide) | 40 g |
| Ethanol (C₂H₅OH) 95% | 100 mL |
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterStrain™ KOH | 06004 | 100mL/ dropper bottle or as per customer request. |
Related products
BacterBagTM Dry Alkaline Saline Peptone Water (ASPW)
- High-quality dry medium, packaged in a transparent, lightweight, and convenient plastic bag. Media used for the growth and enrichment of Vibrio bacteria.
- Code: 10024
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterTest™ Kovac
- Kovacs’ Indole Reagent is used for detection of presence of indole produced by microorganisms due to tryptophan deamination.
- Code: 06003
- Packaging: 100mL/ dropper bottle or as per customer request.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 12 months from the date of manufacture.
BacterContact™ Sabouraud Dextrose Agar + LTHTh
- Ready-to-use medium on 60mm plates for the determination of the total count of yeasts and molds
- Code: 12005
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterContact™ Pro Tryptic Soy Agar
- Ready-to-use medium on 60mm plates for surface microbiological sampling
- Code: 12023
- Packaging: Three-layer packaging irradiated with 10 discs per box, or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 9 months from the date of manufacture.
BacterStain™ Alcohol Acid
- A solution used in microbiological staining methods, particularly in the Ziehl Neelsen staining method.
- Code: 04009
- Packaging: 100ml/bottle or according to the customer’s request..
- Storage Conditions: 18 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
BacterBagTM Dry Tryptone Soya Broth (TSB)
- High-quality dry medium, packaged in a transparent, lightweight, and convenient plastic bag. A highly nutritious general purpose medium for the cultivation of a wide variety of micro-organisms. For identification of Salmonella species from food samples in accordance with FDA BAM.
- Code: 10019
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterTube™ Methyl Red-Voges Proskauer (MR-VP)
- Test medium used for the identification of members of the Enterobacteriaceae family
- Code: 08033
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
BacterBagTM Dry Maximum Recovery Diluent
- High-quality dry medium, packaged in a transparent, lightweight, and convenient plastic bag. Protective and isotonic medium used for maximal recovery of microorganisms from a variety of sources.
- Code: 10016
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterPlate™ TCBS Agar
- Ready-to-use medium on 90mm plates for isolating and selecting Vibrio. Differentiates Vibrio cholerae from other Vibrio species based on sucrose fermentation capability.
- Code: 05022
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.