Vn
INTENDED USE
- BacterTest™ KOH is one of the two main reagents (along with KOH) in the Voges-Proskauer method, playing an important role in creating the alkaline environment necessary for acetoin (or 3-hydroxybutanone) in the sample to react with α-Naphthol, forming a red or pink complex.
PRINCIPLES
- BacterTest™ KOH is one of the two main reagents in this test, with an important role in the detection of acetoin.
- Acetoin (or 3-hydroxybutanone) is produced during the fermentation of glucose (or other carbohydrates) by some species of bacteria.
- When the culture medium contains acetoin, the α-Naphthol reagent will react with acetoin, forming a red or pink complex.
- KOH is necessary to increase the alkalinity of the medium, helping acetoin to convert into substances that can react with α-Naphthol, creating a recognizable color.
- Bacteria are cultured in prepared MR-VP medium after 24 – 48 hours at 37°C (to allow bacteria time to ferment glucose in the medium).
- Adding 0.5 mL of 40% KOH solution to the sample.
- Then, adding 3 – 4 drops of α-Naphthol reagent to the cultured bacteria sample.
- Reacting within minutes to hours.
- Observing the color change of the medium, reading and recording the results.
RESULTS
- Observe the color change of the environment:
- Red or pink = Positive (Bacteria that produce acetoin)
- Colorless or Yellow = Negative (Bacteria do not produce acetoin).
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| REFERENCE STRAINS | CULTURE CONDITIONS | Α-NAPHTHOL + KOH |
| Culture conditions: 18 – 24 hours, incubated at 35 – 37°C, inoculum ≤ 102 CFU/mL | ||
| K. pneumonia NCTC 13442 | Good growth | (–): Red |
| E. coli ATCC 35218 | Good growth | (+): Yellow or no change |
Refer to the components.
| KOH (kali hydroxide) | 40 g |
| Ethanol (C₂H₅OH) 95% | 100 mL |
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterStrain™ KOH | 06004 | 100mL/ dropper bottle or as per customer request. |
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