Vn
INTENDED USE
- BacterTube™ Bile Esculin Agar (BEA) is recommended for use as a differential medium in the isolation and presumptive identification of enterococci/ group D streptococci.
PRINCIPLES
- BacterTube™ Bile Esculin Agar (BEA) contains esculin, ferric citrate to provide ferric ions, and 4% oxbile to inhibit most other strains of nongroup D streptococci. Esculin is hydrolyzed by group D streptococci to form dextrose and esculetin. This compound reacts with the ferric ions contained within the medium, turning the medium from its original amber color to a dark brown to black. Thus the tolerance to the presence of oxbile and the hydrolysis of esculin provide the means to presumptively identify group D streptococci.
- Allow the BacterTube™ Bile Esculin Agar (BEA) warm to room temperature before use.
- Inoculate and streak the medium with one isolated pure colony.
- Incubate in an aerobic atmosphere at 35ºC for 24-48 hours.
- Observe for growth and blackening of the medium.
NOTE:
- This product is not intended for primary isolation of patient specimens. It should be used only with cultures of isolated organism.
- This product is used in conjunction with other biochemical tests to identify cultures of isolated organism.
RESULTS
- The enterococci appear as small translucent colonies surrounded by a black halo.
- Staphylococci and yeasts may yield opaque colonies without a black halo.
- It is indispensable to identify suspected bacteria, especially to eliminate confusion with Listeria which may give rise to colonies similar to those of enterococci.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference Strains | Incubation Conditions | Expected Results |
| Enterococcus faecalis ATCC 29212 | 35 – 37°C
18 – 24 hours, aerobic |
Good growth, black zone around the colony |
| Streptococcus pyogenes ATCC 19615 | Inhibited, no growth |
For 1 liter of medium
| Tryptone | 17,0 g |
| Peptic digest of meat | 3,0 g |
| Yeast extract | 5,0 g |
| Bacteriological ox bile | 10,0 g |
| Sodium chloride | 5,0 g |
| Esculin | 1,0 g |
| Ferric ammonium citrate | 0,5 g |
| Sodium azide | 0,15 g |
| Agar | 13,0 g |
pH of the ready-to-use medium at 25°C: 7,1 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ Bile Esculin Agar (BEA) | 08031 | 50 tubes/ box or as per customer request. |
Related products
BacterTube™ Methyl Red-Voges Proskauer (MR-VP)
- Test medium used for the identification of members of the Enterobacteriaceae family
- Code: 08033
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
BacterContact™ Plate Count Agar
- Ready-to-use medium on 60mm plates for surface microbiological sampling
- Code: 12010
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterStain™ Gram Stain Kit
- A set of reagents used in a technique for the differentiation of Gram-negative and Gram-positive microorganisms.
- Code: 04001
- Packaging: Crystal Violet; Lugol; Safranin; Alcohol: 4 bottles/box 100ml or as per customer request.
- Storage Conditions: 18–25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
BacterPlate™ XLD Agar
- Ready-to-use medium on 90mm plates for detecting Salmonella and Shigella.
- Code: 05043
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
BacterPlate™ Salmonella Shigella Agar (SS)
- Ready-to-use medium on 90mm plates for the selective culture of Salmonella and Shigella. Applied for culturing stool samples.
- Code: 05019
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterBagTM Dry Maximum Recovery Diluent
- High-quality dry medium, packaged in a transparent, lightweight, and convenient plastic bag. Protective and isotonic medium used for maximal recovery of microorganisms from a variety of sources.
- Code: 10016
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterPlate™ Cetrimide Agar
- Ready-to-use medium on 90mm plates for detecting and quantifying Pseudomonas aeruginosa.
- Code: 05031
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
BacterTube™ Lysin decarboxylase (LDC)
- The test medium used to differentiate Salmonella arizona from the Bethesda Ballerup group of Enterobacteriaceae.
- Code: 08030
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
BacterContact™ Pro Tryptic Soy Agar + LT
- Ready-to-use medium on 60mm plates for surface microbiological sampling with the addition of a neutralizer to neutralize surface disinfectants
- Code: 12014
- Packaging: Three-layer packaging irradiated with 10 discs per box, or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 9 months from the date of manufacture.
BacterContact™ Pro Tryptic Soy Agar
- Ready-to-use medium on 60mm plates for surface microbiological sampling
- Code: 12023
- Packaging: Three-layer packaging irradiated with 10 discs per box, or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 9 months from the date of manufacture.