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BacterTube™ BHI Broth
- A nutrient-rich medium that provides essential substances for microbial growth, suitable for culturing various types of microorganisms.
- Code: 08009
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
INTENDED USE
- BacterTube™ BHI Broth is a buffered nutrient medium designed for the culture of aerobic and anaerobic microorganisms, including yeasts and molds. It is also used for the detection of staphylocoagulase and complies with NF EN ISO 6888-1, NF EN ISO 6888-3, and NF V08-057-1 standards in food microbiology.
PRINCIPLES
- BacterTube™ BHI Broth derived from the Rosenow formula (1919), contains:
- Pork brain-heart infusion: Rich in nutritive compounds to support fastidious bacterial growth.
- Gelatin digest: Supplies amino acids and peptides.
- Sodium chloride: Maintains osmotic balance.
- Disodium phosphate: Buffers pH stability.
- Glucose: Provides an energy source to enhance growth
Confirmation of Coagulase-Positive Staphylococci (NF EN ISO 6888-1 & 3):
- Re-isolate a characteristic colony into a BacterTube™ BHI Broth.
- Incubate at 35 – 37°C for 24 hours.
- Proceed with the coagulase tube test using Coagulase Rabbit Plasma
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Staphylococcus aureus ATCC 25923 | 24 hours, 36°C | Growth causing turbidity in the culture medium |
| Escherichia coli ATCC 25922 | Growth causing turbidity in the culture medium |
For 1 liter of medium
| Brain infusion solids | 12,5 g |
| Beef heart infusion solids | 5,0 g |
| Proteose peptone | 10,0 g |
| Sodium chloride | 5,0 mL |
| Disodium phosphate | 2,5 g |
pH of the ready-to-use medium at 25°C: 7,4 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ BHI Broth | 08009 | 50 tubes/ box or as per customer request. |
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