BacterTube™ Motility Test Medium

INTENDED USE

BacterTube™ Motility Test Medium is used to test the motility of microorganisms.

PRINCIPLES

BacterTube™ Motility Test Medium is designed to detect flagellated bacteria, as their motility is demonstrated by growth spreading beyond the initial stab line in the semi-solid agar medium.

This medium is not for primary isolation; use isolated colonies from an 18 – 24 hour culture
Perform the test in tubes for Enterobacteriaceae, non-fermenting Gram-negative bacilli, and Listeria.
Using a sterile inoculating needle, pick a pure colony and inoculate the semi-solid agar in a straight line through the center, penetrating ½ inch
Incubate Enterobacteriaceae at 35°C for 24 hours.
Incubate non-fermenting Gram-negative bacilli and Enterococci at 30°C for 24 hours.
If negative results are suspected, incubate at 25°C for confirmation.
For Listeria and Yersinia, inoculate two tubes: incubate one at 35°C and the other at 25°C.

RESULTS

Positive Test: Bacteria spread out from the stab line or cause turbidity in the medium.
Non-motile Bacteria: The tube remains clear (similar to the uninoculated medium) with bacterial growth confined to the stab line.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Escherichia coli ATCC 25922	18 – 24 hours, 35 – 37^oC	Good growth: Bacteria spread out from the stab line.
Klebsiella pneumonia ATCC 13883	18 – 24 hours, 35 – 37^oC	Good growth: Bacteria remain confined to the stab line

For 1 liter of medium

pH of the ready-to-use medium at 25°C: 7,0 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterTube™ Motility Test Medium	08038	50 tubes/ box or as per customer request.