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INTENDED USE
- BacterTube™ Peptone Diluent The preliminary enrichment medium in the process of isolating bacteria from food samples is commonly as a diluent for preparing samples before further testing.
PRINCIPLES
- BacterTube™ Peptone Diluent is a diluent solution combined with the support of another substance to maximize the recovery of microorganisms. Peptone in the diluent solution serves a protective role. The low concentration of peptone does not cause microbial growth within 45 minutes (at 20-25°C) after the sample dilution.
- Prior to inoculation, allow medium to equilibrate to room temperature
- Use a sterile inoculation loop to transfer a sample from an isolated colony on the agar plate into the test tube containing the medium.
- Incubate the tube at 35 – 37°C.
- Examine the tube after 18 – 24 hours of incubation.
- Observe and record the results.
RESULTS
- Based on the turbidity of each tube, the growth ability of the strain can be determined.
- To identify the bacterium, further appropriate tests must be carried out.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| S. aureus ATCC 25923 | 18 – 24 hours, 35 – 37oC | Good growth |
| Escherichia coli ATCC 25992 | Good growth |
For 1 liter of medium
| Peptone | 1,0 g |
| Distilled water | 1,0 L |
pH of the ready-to-use medium at 25°C: 7,0 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ Peptone Diluent | 08041 | 50 tubes/ box or as per customer request. |
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