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BacterTube™ Pyruvate Broth
- The test medium is used to determine the organism’s ability to utilize the substrate pyruvate.
- Code: 08034
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
INTENDED USE
- BacterTube™ Pyruvate Broth is recommended for the differentiation of microorganisms based on pyruvate utilization.
PRINCIPLES
- The medium supports microbial differentiation through the following components:
- Sodium Pyruvate: Serves as the fermentable carbohydrate.
- Bromothymol Blue: An acid-base indicator that turns yellow when acid is produced during fermentation.
- Yeast Extract: Provide essential nutrients for growth.
- Diphosphate Buffer: Maintains pH stability throughout incubation
- Prior to inoculation, allow medium to equilibrate to room temperature.
- Inoculate with 2 – 3 colonies from a 18 – 24 hour culture.
- Incubate aerobically at 35ºC for up to 5 days.
- Examine daily for a yellow reaction.
RESULTS
- Pyruvate fermentation is indicated by the appearance of a yellow color in the broth within 5 days following inoculation.
- Negative result when the medium does not change color.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Enterococcus faecalis ATCC 29212 | After 48 hours, 35 – 37oC | Growth with yellow color change |
| Enterococcus faecium ATCC 6569 | Growth with no color change | |
| Streptococcus bovis ATCC 9809 | Growth with no color change |
For 1 liter of medium
| Pancreatic digest of casein | 10,0 g |
| Yeast extract | 5,0 g |
| Sodium chloride | 5,0 g |
| Dipotassium phosphate | 5,0 g |
| Bromthymol blue | 0,04 g |
| Sodium pyruvate | 10,0 g |
pH of the ready-to-use medium at 25°C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ Pyruvate Broth | 08034 | 50 tubes/ box or as per customer request. |
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