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BacterTube™ Sterile Physiological Saline (NS 0,85%)
- Solution used for sample dilution and preparation of bacterial suspensions for identification and antibiotic susceptibility testing
- Code: 08019
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
INTENDED USE
- BacterTube™ Sterile Physiological Saline (NS 0,85%) is recommended for procedures that require the use of an isotonic diluent. It is also recommended foruse in preparing test suspensions of organisms
PRINCIPLES
- Saline is useful as a diluent to maintain cell integrity andviability because it lacks properties that may interfere with biochemical reactions and/or antibiotic susceptibility tests. The concentration of sodium chloride in 0.85% (normal) Saline provides osmotic protection for microbial cells. Normal saline is used for preparing microbial suspensions when it is necessary to deliver a setnumber of microbes to an identification test battery, to antimicrobial agents, or to growth media used for disk susceptibility testing. It is also used in the preparing of stock solutions and serial dilutions of antimicrobial agents. The Clinical and Laboratory Standards Institute (CLSI – formerly NCCLS) recommends the use of 0.85% Saline to adjustthe turbidity of bacterial suspensions to help maintain cell integrityand viability.
- Solutions for inoculation should be prepared by inoculating the diluent with one to five isolated colonies of test organism.
- The suspension should be adjusted to the concentration of the appropriate McFarland inoculum standard.
| Criteria | Incubation conditions | Expected results |
| Sterility | 18 – 24 hours, 35 – 37°C | Free from microbial contamination |
For 1 liter of medium
| Sodium chloride | 8,5 g |
pH of the ready-to-use medium at 25°C: 5,5 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ Sterile Physiological Saline (NS 0,85%) | 08019 | 50 tubes/ box or as per customer request. |
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