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BacterChrom™ Pseudomonas Plates
- Ready-to-use chromogenic medium on 90mm plates for the detection and isolation of Pseudomonas spp.
- Code: 01042
- Packaging: 10 plates/box or as per customer requirem ents.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ Pseudomonas is a chromogenic medium designed for the isolation and detection of Pseudomonas species. It is particularly useful in environmental and food safety testing, as Pseudomonas aeruginosa serves as an indicator of disinfection efficacy in recreational water systems such as swimming pools and wading pools. The medium also aids in identifying Pseudomonas as an opportunistic pathogen often associated with waterborne transmission.
- Results are observable after 24 – 36 hours of aerobic incubation at 30 °C, with an extension to 48 hours for fragile Pseudomonas strains. For targeted detection of P. aeruginosa, incubation at 37°C or 41°C is recommended based on the application field.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ Pseudomonas employs chromogenic substrates that react with specific enzymatic activities of target microorganisms, producing distinct colony colors for easy differentiation. Selective agents are incorporated to inhibit the growth of non-target organisms, ensuring high specificity. This combination enables reliable detection and simplifies the identification process in various sample types.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Apply the sample onto the plate either by direct inoculation or by evenly spreading it across the surface. If using the filtration technique, gently place the inoculated membrane onto the plate’s surface
- Incubate in aerobic conditions at 30°C for 24 – 36 hours.
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| Pseudomonas spp. | blue green |
| Most of Enterobacteriaceae | mauve to violet or inhibited |
| Gram (+) bacteria | inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| P. aeruginosa ATCC 9027 | Incubate for 24 – 36 hours at 35 – 37°C | Blue green colonies |
| K. pneumoniae NCTC 13442 | Violet colonies | |
| E. faecalis ATCC 29212 | Inhibited | |
| E. coli ATCC 35218 | Inhibited | |
| S. aureus ATCC 25923 | Inhibited |
For 1 liter of medium
| Peptone | 20,0 g |
| Chromogenic mix và selective | 2,5 g |
| Agar | 15,0 g |
| Salt | 8,0 g |
pH of the ready-to-use medium at 25°C: 7,5 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ Pseudomonas | 01042 | 10 plates/box or as per customer request. |
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