Vn
INTENDED USE
- BacterChrom™ Acinetobacter is a selective and differential chromogenic culture medium intended for the direct qualitative detection of Acinetobacter colonization. This medium aids in infection prevention and control by screening samples such as rectal swabs, nare swabs, wound swabs, stool, and urine from patients in healthcare settings. It can also be utilized for surface sampling to monitor hygiene in clinical environments.The medium also serves as an early warning indicator for diagnostic tests by identifying the possible presence of multidrug-resistant (MDR) bacteria.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ Acinetobacter employs chromogenic substrates that react with specific enzymatic activities of Acinetobacter species, producing distinct colony colors for easy identification. The medium contains selective agents that suppress the growth of non-target organisms, ensuring specificity and minimizing background interference. When combined with the optional MDR selective supplement, the medium enhances the detection of multidrug-resistant Acinetobacter strains.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Direct streaking sample onto the plate
- Incubate at 35 – 37 °C for 18 – 24 hours in aerobic conditions.
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| Acinetobacter sp. | Red |
| Other Gram (-) | Mostly inhibited or blue |
| Gram (+) bacteria & yeasts | Mostly inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| A. baumannii ATCC BAA747 | Incubate for 18 – 24 hours at 35 – 37 °C | Red colonies |
| A. baumannii ATCC BAA1605 | Red colonies | |
| E. faecalis ATCC 29212 | Inhibited | |
| C. tropicalis ATCC 1369 | Inhibited |
For 1 liter of medium
| Peptone | 12,0 g |
| Sodium chloride | 5,0 g |
| Chromogenic mix | 1,8 g |
| Agar | 15,0 g |
| Growth factor | 4 mL |
pH of the ready-to-use medium at 25°C: 7,0 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ Acinetobacter | 01024 | 10 plates/box or as per customer request. |
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