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BacterTube™ Malonate broth
- The test medium is used to differentiate Enterobacter and Escherichia based on malonate utilization.
- Code: 08035
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
INTENDED USE
- BacterTube™ Malonate Broth h is recommended for use in the differentiation of Enterobacteriaceae on the basis of malonate utilization.
PRINCIPLES
- The medium distinguishes malonate-positive organisms (e.g., Klebsiella pneumoniae) from malonate-negative organisms (e.g., Escherichia coli).
- Malonate and Ammonium Sulfate: Organisms that utilize both produce sodium hydroxide, causing alkalinity and a color change to light blue or Prussian blue.
- Bromothymol Blue: pH indicator; turns blue in alkaline conditions and yellow in acidic environments due to glucose fermentation.
- Yeast Extract and Dextrose: Provide minimal growth for organisms unable to utilize malonate
- Prior to inoculation, allow medium to equilibrate to room temperature
- Using a light inoculum from an 18 – 24 hour pure culture, inoculate the tube.
- Incubate the tube with loosened caps at 35ºC in an aerobic atmosphere for 24 – 48 hours
- Observe for alkalization (blue color) at 24 and 48 hours
RESULTS
- Positive Test: Blue color indicates malonate utilization.
- Negative Test: Green or yellow color (due to glucose fermentation)
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Klebsiella pneumoniae ATCC 13883 | 24 – 48 hours, 35 – 37oC | Positive: Good growth, blue color |
| Escherichia coli ATCC 25992 | Negative: Inhibited, light green color |
For 1 liter of medium
| Ammonium sulphate | 2,0 g |
| Dipotassium phosphate | 0,6 g |
| Monopotassium phosphate | 0,4 g |
| Sodium chloride | 2,0 g |
| Sodium malonate | 3,0 g |
| Bromothymol blue | 0,025 g |
pH of the ready-to-use medium at 25°C: 6,7 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ Malonate Broth | 08035 | 50 tubes/ box or as per customer request. |
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