BacterTest™ Kovac

Kovacs’ Indole Reagent is used for detection of presence of indole produced by microorganisms due to tryptophan deamination.
Code: 06003
Packaging: 100mL/ dropper bottle or as per customer request.
Storage Conditions: 8 – 25°C.
Transportation: Ambient temperature.
Shelf Life: 12 months from the date of manufacture.

INTENDED USE

BacterTest™ Kovac is a reagent used in the Indole Test reaction to determine the ability of bacteria to decompose tryptophan into indole, an organic compound that has a characteristic color when reacting with Kovac’s reagent.

PRINCIPLES

BacterTest™ Kovac is based on the ability of certain bacteria to break down tryptophan into indole.
- Bacteria break down tryptophan into indole using the enzyme tryptophanase.
- Kovac’s reagent (p-Dimethylaminobenzaldehyde, HCl and isoamyl alcohol) reacts with indole to produce a red or pink color, which is an indication of a positive reaction (bacteria producing indole).

Bacteria are cultured in a prepared medium containing tryptophan (usually Tryptone Broth) after 24 – 48 hours at 37°C.
Adding 1 mL of Kovac’s reagent to the bacterial culture medium.
Observing the color change of the medium, reading and recording the results.

RESULTS

Observe the color change of the environment:
- Red = Positive (indole producing bacteria)
- Colorless or Yellow = Negative (bacteria do not produce indole).

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Refer to the components.

p-Dimethylaminobenzaldehyde (DMAB)	50 g
Isoamyl alcohol (hoặc butanol)	750 mL
Axit clohydric, 37%	250 mL

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterStrain™ Kovac	06003	100mL/ dropper bottle or as per customer request.