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BacterTest™ Kovac
- Kovacs’ Indole Reagent is used for detection of presence of indole produced by microorganisms due to tryptophan deamination.
- Code: 06003
- Packaging: 100mL/ dropper bottle or as per customer request.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 12 months from the date of manufacture.
INTENDED USE
- BacterTest™ Kovac is a reagent used in the Indole Test reaction to determine the ability of bacteria to decompose tryptophan into indole, an organic compound that has a characteristic color when reacting with Kovac’s reagent.
PRINCIPLES
- BacterTest™ Kovac is based on the ability of certain bacteria to break down tryptophan into indole.
- Bacteria break down tryptophan into indole using the enzyme tryptophanase.
- Kovac’s reagent (p-Dimethylaminobenzaldehyde, HCl and isoamyl alcohol) reacts with indole to produce a red or pink color, which is an indication of a positive reaction (bacteria producing indole).
- Bacteria are cultured in a prepared medium containing tryptophan (usually Tryptone Broth) after 24 – 48 hours at 37°C.
- Adding 1 mL of Kovac’s reagent to the bacterial culture medium.
- Observing the color change of the medium, reading and recording the results.
RESULTS
- Observe the color change of the environment:
- Red = Positive (indole producing bacteria)
- Colorless or Yellow = Negative (bacteria do not produce indole).
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Growth | Indole test |
| E. coli ATCC 35218 | Good growth | (+): red |
| K.aerogenes ATCC 13048 | Good growth | (–):Colorless or Yellow |
Refer to the components.
| p-Dimethylaminobenzaldehyde (DMAB) | 50 g |
| Isoamyl alcohol (hoặc butanol) | 750 mL |
| Axit clohydric, 37% | 250 mL |
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterStrain™ Kovac | 06003 | 100mL/ dropper bottle or as per customer request. |
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