BacterStain™ Giemsa

A dye used for microbiology and hematology, commonly employed for staining cell and tissue samples in bacteriological, virological, and hematological studies.
Code: 04003
Packaging: 100ml/bottle or according to the customer’s request..
Storage Conditions: 18–25°C.
Transportation: Ambient temperature.
Shelf Life: 24 months from the date of manufacture.

INTENDED USE

BacterStrain™ Giemsa is a stain primarily used for staining blood cells and microorganisms. It helps differentiate various cell types in hematological examinations, as well as staining parasites such as Plasmodium (the causative agent of malaria) in microbiological studies.

PRINCIPLES

BacterStain™ Giemsa works by binding to the DNA and proteins within cells, producing distinct colors that allow differentiation of cell components. In blood samples, red blood cells and white blood cells stain differently, making it easier to identify cell types. The stain also highlights parasites such as Plasmodium within red blood cells.

Prepare the sample: Spread the blood or cell sample onto the slide, let it dry, and fix the sample with 95% methanol for 5-10 minutes.
Dilute 5 mL of Giemsa stock solution with 65 mL of distilled water to make the staining solution.
Rinse the slide in distilled water about 15 times to remove impurities and excess solution.
Immerse the slide in the diluted Giemsa solution for 2 hours to allow the dye to penetrate the cells.
Dip the slide into a 1% acetic acid solution once to brighten the dye.
Blot dry the glass slide gently with tissue paper.
Immerse the slide in 100% ethanol until the solution runs out with only a faint blue color remaining.
Dip the slide into xylene I solution about 10 times to remove impurities and alcohol solvent.
Continue dipping the slide into xylene II solution about 10 times for further cleaning.
Mount the coverslip with Permount.

RESULTS

BacterLab quality control of each batch of products.

STT	Evaluation criteria	Result
1	Specimen size	Size 1x2cm, oval owl located in the middle of the glass slide
2	Smoothness	The specimen surface is continuous, even, not hollow or bare. Microscopic examination: Continuous microscopic fields without many empty microscopic fields, uniform brightness.
3	Thickness	Standard specimens have a thickness of about 0.04 mm. When the specimen is dry and not stained, it can be checked by placing a piece of paper with printed letters 4-5 cm below the specimen. If the letters are faint and readable, it is qualified. If the letters are illegible, it is thick. If the letters are too clear, it is thin.