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BacterPlate™ Horse Blood Agar (HBA)
- Ready-to-use medium on 90mm plates used for the non-selective culture of fastidious bacteria (Haemophilus, Neisseria spp).
- Code: 05007
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Horse Blood Agar (HBA) is an enriched, non-selective medium used for the isolation, cultivation, and differentiation of fastidious microorganisms, including Streptococcus spp. and Haemophilus spp.
- The typical composition of base medium (Columbia Agar) corresponds to that defined in European (EP), United States (UP) and Japanese (JP) Pharmacopeias.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- Peptones included in the composition of the medium favor the excellent growth of colonies.
- Yeast extract is a source of vitamin B complex.
- Starch is a detoxifying agent and also an energy source.
- The addition of sterile defibrinated horse blood enables the observation of hemolytic activity.
- Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
- Inoculate in order to obtain isolated colonies.
- Incubate at 37°C, 5 – 10% CO2 for 24 to 48 hours in optimal conditions for the culture of the inoculated germs.
RESULTS
- After incubation for the required duration, observe the colonies growing on the surface of the plate for characteristic traits such as colony size, color, and morphology.
- Helicobacter pylori: Small, round, translucent colonies with a smooth appearance, often greyish-white
- Contaminants (Gram-positive, Gram-negative bacteria, or fungi) should be inhibited by the VTAP selective agents.
- Identification of the isolated bacteria must be carried out through appropriate subsequent tests
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Microbial Standard and Incubation Conditions | Standard (PR) |
| Incubation conditions after 24 – 48 hours, at 35 – 37°C, 5 – 10% CO2 with inoculum ≤ 103 – 104 CFU/ mL | |
| H. influenzae ATCC 33533 | Good growth |
| S. pyogenes ATCC 19615 | Good growth, |
For 1 liter of medium
| Special peptone | 23,0 g |
| Starch | 1,0 g |
| Sodium chloride | 5,0 g |
| Agar | 13,5 g |
| Defibrinated horse blood | 50 – 70 mL |
pH of the ready-to-use medium at 25°C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ Horse Blood Agar (HBA) | 05007 | 10 plates/ box or as per customer request. |
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