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BacterPlate™ Chocolate Agar with Gentamycin
- Ready-to-use medium for culturing fastidious microorganisms, particularly Neisseria and Haemophilus.
- Code: 05015
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Chocolate Agar with Gentamycin is a ready-to-use selective medium designed for the isolation and cultivation of fastidious Gram-negative bacteria, particularly Haemophilus and Neisseria species. The addition of gentamicin enhances selectivity by inhibiting the growth of contaminating bacteria, making it ideal for diagnostic workflows in clinical microbiology laboratories.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterPlate™ Chocolate Agar with Gentamycin is formulated with:
- Defibrinated Blood: Heat-treated to release growth factors (hemin and NAD), essential for the growth of Haemophilus and Neisseria
- Gentamycin: Acts as a selective agent to inhibit unwanted bacterial contaminants.
- Enriched Nutrients: Supports the robust growth of target organisms.
- This combination promotes the selective recovery and reliable differentiation of fastidious pathogens, ensuring efficient and accurate diagnostic results.
- Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
- Inoculate in order to obtain isolated colonies.
- Incubate at 35 – 37 °C, 5 – 10% CO2 for 18 – 24 hours in optimal conditions for the culture of the inoculated germs.
RESULTS
- After incubation for the required duration, observe the colonies growing on the surface of the plate for characteristic traits such as colony size, color, and morphology.
- Haemophilus influenzae: Smooth, greyish, translucent colonies.
- Neisseria gonorrhoeae: Small, shiny, grey colonies with a mucoid appearance.
- Identification of the isolated bacteria must be carried out through appropriate subsequent tests
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation Conditions | Expected results |
| H. influenzae ATCC 33533 | Incubate for 18 – 24 hours, 35 – 37°C, 5–10% CO2, | Good growth |
| S. pneumoniae ATCC 49619 | Weak growth or no growth | |
| N. meningitidis ATCC 13090 | Weak growth or no growth |
For 1 liter of medium
| Polypeptone | 23,0 g |
| Starch | 1,0 g |
| Sodium chloride | 5,0 g |
| Defibrinated horse blood | 50 – 70 mL |
| Gentamycin | 0,008 g |
| Agar | 13,5 g |
pH of the ready-to-use medium at 250C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ Chocolate Agar with Gentamycin | 05015 | 10 plates/ box or as per customer request. |
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