BacterPlate™ Two-Compartment Medium BA/CAHI

Ready-to-use medium on 90mm plates for the cultivation and isolation of infectious bacteria from respiratory sample specimens.
Code: 05045
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 04 months from the date of manufacture.

INTENDED USE

BacterPlate™ Two-Compartment Medium BA/CAHI is a nutrient-rich and selective culture medium used for isolating Haemophilus influenzae and other fastidious bacteria from clinical specimens. The medium combines Blood Agar (BA) and Chocolate Agar with Bacitracin (CAHI), which helps inhibit the growth of Gram-positive bacteria and non-target Gram-negative bacteria, while optimizing the recovery of H. influenzae. This makes it an ideal medium for diagnostic microbiology laboratories.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterPlate™ Two-Compartment Medium BA/CAHI:

BA (Blood Agar) Compartment: This is a blood-based culture medium that helps differentiate bacteria based on their hemolytic properties. Hemolytic reactions can be categorized into three types: beta (β) hemolysis, alpha (α) hemolysis, and gamma (ɤ) hemolysis. These hemolytic reactions help identify the characteristics of bacteria such as Streptococcus and Listeria.
CAHI (Chocolate Agar with Bacitracin) Compartment: This medium helps isolate Haemophilus influenzae by inhibiting the growth of other bacteria through the presence of Bacitracin, an antibiotic that selectively targets Gram-positive bacteria and some Gram-negative bacteria.

Allow the agar plates to reach room temperature before use.
Dry the agar plates in an incubator with the lid slightly open.
Inoculate the sample by spreading it evenly on the surface of the agar using a sterile inoculating loop.
Incubate at 35–37°C for 18–24 hours.

RESULTS

BA Compartment:

Beta Hemolysis (β): Colonies will appear with a complete hemolytic zone (clear zone) around them. Beta-hemolytic bacteria include Streptococcus pyogenes and Listeria.
Alpha Hemolysis (α): Colonies will have a narrow, greenish or faint hemolytic zone. Alpha-hemolytic bacteria include Streptococcus pneumoniae.
Gamma Hemolysis (ɤ): No hemolytic zone, e.g., Enterococcus faecalis.

CAHI Compartment:

Haemophilus influenzae: Smooth, light gray, transparent colonies.
Other bacteria: The growth of non-H. influenzae bacteria will be inhibited by Bacitracin.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.