BacterPlate™ Two-Compartment Medium Edwardsiella/Aeromonas

Ready-to-use medium on 90mm plates for the detection and differentiation of Edwardsiella ictaluri and Aeromonas hydrophila
Code: 05052
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 04 months from the date of manufacture.

INTENDED USE

BacterPlate™ Two-Compartment Medium Edwardsiella / Aeromonas is a ready-to-use two-compartment agar medium designed for the detection and differentiation of Edwardsiella and Aeromonas bacteria. The product is applied in microbiological research, clinical diagnostics, and microbiological quality control of food, water, and animal-derived biological samples.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterPlate™ Two-Compartment Medium Edwardsiella/Aeromonas:

Edwardsiella Compartment: This compartment contains special components that help isolate Edwardsiella bacteria, a Gram-negative bacterium that causes infections in animals, including humans. The medium stimulates the growth of Edwardsiella strains, helping differentiate them from other bacteria based on their ability to ferment various sugars and using special color indicators.
Aeromonas Compartment: This selective medium contains specific components to isolate and identify Aeromonas strains, a Gram-negative bacterium primarily responsible for diseases in aquatic environments, causing diarrhea and septicemia. Aeromonas bacteria will grow strongly and can be differentiated from other bacteria through fermentation reactions and color indicators.

Allow the agar plates to reach room temperature before use.
Dry the agar plates in an incubator with the lid slightly open.
Inoculate the sample by spreading it evenly on the surface of the agar using a sterile inoculating loop.
Incubate at 35–37°C for 18–24 hours.

RESULTS

Edwardsiella Compartment

Edwardsiella (+): Colonies grow well, with a characteristic color, and may show a sugar fermentation reaction, depending on the sample type.
Edwardsiella (–): No growth or weak growth.

Aeromonas Compartment

Aeromonas (+): Colonies grow strongly, with a characteristic color, and may show a sugar fermentation reaction.
Aeromonas (–): No growth or weak growth.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Edwardsiella Compartment
Edwardsiella ictaluri ATCC 33202	Incubate at 35–37°C for 18–24 hours.	Good growth, green colonies
Edwardsiella tarda NCIMB 2056		Good growth, green colonies
Serratia marcescens ATCC 8100		Good growth, yellow or red colonies
Proteus mirabilis ATCC 29906		Good growth, gray colonies
Aeromonas Compartment
E. coli IMP NCTC 13476	Incubate at 35–37°C for 18–24 hours.	Good growth, pink colonies with black center
K. pneumoniae ATCC BAA 1705		Good growth, metallic greenish colonies
K. pneumoniae NCTC 13438		Good growth, metallic greenish colonies
E. faecalis ATCC 29212		Inhibited
K. pneumoniae ATCC 13883		Inhibited
S. aureus ATCC 25293		Inhibited
C. albicans ATCC 10231		Inhibited

For 1 liter of Edwardsiella medium

pH of the ready-to-use medium at 25°C: 7,1 ± 0,2

For 1 liter of Aeromonas medium

pH of the ready-to-use medium at 25°C: 7,0 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterPlate™ Two-Compartment Medium Edwardsiella/Aeromonas	05052	10 plates/ box or as per customer request.