BacterPlate™ Mannitol Salt Agar (MSA)

Ready-to-use medium on 90mm plates for the selective culture of Staphylococcus, differentiating between Staphylococcus aureus and Staphylococcus epidermidis.
Code: 05021
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 04 months from the date of manufacture.

INTENDED USE

BacterPlate™ Mannitol Salt Agar (MSA) is used for the selective isolation, detection and enumeration of pathogenic staphylococci in filterable water as in swimming pools, potable water or spas. It is also used for the detection of Staphylococcus aureus according to the Pharmacopeia and in cosmetic products.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

The high sodium chloride concentration inhibits the growth of most bacteria other than staphylococci.
Mannitol fermentation, shown by the color change of the pH indicator (phenol red) to yellow orients the diagnosis.
The demonstration of pathogenic staphylococci is generally confirmed by a coagulase test.

1. Detection of Staphylococcus aureus (Pharmacopeia, NF EN ISO 22718)

2. Water testing by membrane filtration

Aseptically filter the appropriate volume of water through a nitrocellulose membrane.
Place the membrane, filtered side up, on the surface of the plates, insuring a complete contact.
Incubate at (36 ± 2)°C for (44 ± 4) hours.

RESULTS

Count the characteristic colonies.
Pathogenic staphylococci form luxuriant pigmented colonies, surrounded by a yellow ring due to the fermentation of mannitol.
Non-pathogenic staphylococci generally give rise to small red colonies which do not change the color of the medium.
Several strains of Staphylococcus epidermidis can ferment mannitol.
After 48 hours of incubation, several strains of enterococci, Bacillus, Micrococcus and Serratia may grow

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.