TraceMedia™ Columbia Sheep/Chocolate Bacitracin

Product code: TM10922.
Packaging options: 10 plates/box; 20 plates/box.
Recommended storage condition: 2 – 8°C.
Transportation temperature: Room temperature.
Shelf life: 03 months from the date of manufacture.

The product “TraceMedia™ Columbia Sheep/Chocolate Bacitracin” is a pre-poured culture medium on 90mm Petri dishes used for the cultivation and isolation of bacterial pathogens from respiratory specimens.

PURPOSE

TraceMedia™ Columbia Sheep/Chocolate Bacitracin is a nutrient-rich dual-compartment culture medium containing Blood Agar (BA) and Chocolate Agar with Bacitracin (CAHI).
BA: This medium is used for cultivation and differentiation of Streptococcus bacterial strains, distinguishing three types of hemolytic reactions (α, β, γ).
CAHI: This selective medium is designed for the isolation of H. influenzae.

PRINCIPLE

TraceMedia™ Columbia Sheep/Chocolate Bacitracin

BA Compartment: The Columbia Agar Base is supplemented with sheep blood, providing essential growth factors and enabling the determination of bacterial hemolytic reactions. On the surface of the blood agar plate, bacterial colonies with clear zones of hemolysis are classified as β-hemolytic. Colonies surrounded by a greenish zone indicate α-hemolysis. Gamma-hemolytic organisms do not produce any hemolytic reactions on the agar surface.
CAHI Compartment: The horse blood in the chocolate agar provides essential factors X and V for the growth of H. influenzae, while the addition of Bacitracin inhibits the growth of Gram-positive bacteria.

INTERPRETATION OF RESULTS

After incubation for the specified time, observe bacterial growth and characteristics on the surface of the culture medium.
Further identification of isolated bacterial strains should be conducted using appropriate tests.

The relevant samples are directly inoculated onto the agar plates.

If the agar plates have been refrigerated, allow them to warm up to room temperature before inoculating the samples.
Inoculate the samples onto the agar plates.
Incubate the plates in an aerobic condition at 35 – 37°C for 16 – 24 hours.

BacterLab conducts quality testing for each batch of products using standard ATCC strains. The results of the culture are as follows:

Microorganism strain	Strain Incubation Conditions	Growth result
Compartment BA
Streptococcus pneumonia ATCC 49619	35 – 37⁰C 5% CO₂, 16–24h	Good growth, α-hemolytic bacteria
Streptococcus pyogenes ATCC 19615		Good growth, β-hemolytic bacteria
Enterococcus faecalis ATCC 29212		Good growth, non-hemolytic bacteria
CAHI Compartment
Haemophilus influenzae ATCC 49766	35 – 37⁰C 5% CO₂, 16–24h	Good growth
Streptococcus pneumonia ATCC 49619		Weak to no growth
Neisseria.meningitidis ATCC 13090		Weak to no growth

In 1 liter of BA (Blood Agar) medium:

For 1 liter of BA medium:
Peptone	10,0 g
Triptose	10,0 g
Sodium chloride	5,0 g
Sheep blood	5-7%
pH of the complete medium at 25°C: 7.4 ± 0.2
For 1 liter of CAHI medium:
Casein Peptone	23,0 g
Meat Peptone	7,5 g
Sodium chloride	5,0 g
Dipotassium Phosphate	4,0 g
Bacitracin	0, 175 g
Horse blood	5-7%
pH of the complete medium at 25°C: 7.2 ± 0.2

*Please note that these compositions are for reference only. Our company can customize the components according to specific requirements or tender documents.