BacterChrom™ Yeast, Mold Agar
- Ready to use chromogenic medium on 90mm plates or quantifying yeasts and molds in food and water.
- Code: 01001
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ Yeast, Mold Agar allows the enumeration of yeasts and molds in all human and animal food products regardless of their water activity. It can also be used for the control of environmental samples in production areas. In the case of water samples, they can be analyzed by membrane filtration using this media.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- The choice of peptones, carbohydrates and growth promotors were specially selected in order to optimize the rapid growth of yeasts & molds.
- Rose Bengal is assimilated by yeasts which facilitate their enumeration by coloring them pink.
- The selective system, associated with the pH of the media, insures the inhibition of the majority of bacterial contaminants.
- The media has been conceived in a way that reduces the propagation of Mucor thallus, which facilitates their count after only 54 hours of incubation. It is also well adapted to the enumeration of mold spores
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Transfer 0,1 mL of the sample to be tested and its serial dilutions to the surface of the ready-to-use plates.
- To estimate low numbers, inoculate 1 mL of initial suspension on to the surface of 3 plates
- Spread the inoculum over the surface of the plate with a sterile triangle or “hockey stick”.
- Incubate the plates with the cover facing up at 25 °C for 54 to 72 hours.
RESULTS
- Count only plates containing less than 150 colonies.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
Reference strains | Incubation conditions | Expected results |
S. cerevisiae ATCC 20827 | Incubate for 72h at 25 ± 1oC | PR ≥ 50 % |
C. albicans ATCC 10231 | PR ≥ 50 % | |
A. brasiliensis ATCC 16404 | PR ≥ 50 % | |
E. coli ATCC 35218 | Inhibited |
For 1 liter of medium
Peptone | 10,0 g |
Glucose | 18,0 g |
Growth promotors | 1,0 g |
Selective system | 1,0 g |
Bacteriological agar | 15,5 g |
pH of the ready-to-use medium at 25°C: 5,6 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
Product name | Code | Packaging |
BacterChrom™ Yeast, Mold Agar | 01001 | 10 plates/ box or as per customer request. |
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