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BacterPlate™ Chocolate Agar (CAXV)
- Ready-to-use medium on 90mm plates for the non-selective culture of fastidious bacteria, particularly those of the Neisseria and Haemophilus families.
- Code: 05009
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Chocolate Agar (CAXV) is a highly enriched culture medium designed for the cultivation and isolation of fastidious microorganisms. It is commonly used in clinical microbiology and microbiological quality control testing. This medium supports the growth of organisms such as Haemophilus, Neisseria, Taylorella, and Campylobacter, which require specific growth factors (X and V factors). The heating process applied to the blood during preparation releases these essential factors, enhancing bacterial growth and facilitating diagnostic applications.
- The typical composition of base medium (Columbia Agar) corresponds to that defined in European (EP), United States (UP) and Japanese (JP) Pharmacopeias.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- Peptones included in the composition of the medium favor the excellent growth of colonies.
- Yeast extract is a source of vitamin B complex.
- Starch is a detoxifying agent and also an energy source.
- The heat treatment of blood in the medium lyses red blood cells, releasing growth factors such as hemin (X factor) and NAD (V factor), which are essential for the growth of certain bacteria.
- Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
- Inoculate in order to obtain isolated colonies.
- Incubate at 37 °C, 5 – 10% CO2 for 24 to 48 hours in optimal conditions for the culture of the inoculated germs.
RESULTS
- After incubation for the required duration, observe the colonies growing on the surface of the plate for characteristic traits such as colony size, color, and morphology.
- Haemophilus influenzae: Smooth, greyish, translucent colonies.
- Neisseria gonorrhoeae: Small, shiny, grey colonies with a mucoid appearance.
- Identification of the isolated bacteria must be carried out through appropriate subsequent tests
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation Conditions | Expected results |
| H. influenza ATCC 33533 | Incubate for 18 – 24 hours, 5–10% CO2, | Good growth |
| S. pneumoniae ATCC 49619 | Good growth | |
| N. meningitidis ATCC 13090 | Good growth |
For 1 liter of medium
| Polypeptone | 23,0 g |
| Starch | 1,0 g |
| Sodium chloride | 5,0 g |
| Defibrinated horse blood | 50 – 70 mL |
| Agar | 13,5 g |
pH of the ready-to-use medium at 250C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ Chocolate Agar (CAXV) | 05009 | 10 plates/ box or as per customer request. |
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