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INTENDED USE
- BacterPlate™ Chocolate Agar with Isovilatex (CAIS) is an enriched and selective culture medium specifically formulated for the isolation of fastidious organisms requiring hemin (X factor), NAD (V factor), and additional nutritional supplements. Isovilatex enhances the growth of fastidious pathogens while reducing interference from contaminants, making this medium ideal for clinical diagnostics and research applications.
- The typical composition of base medium (Columbia Agar) corresponds to that defined in European (EP), United States (UP) and Japanese (JP) Pharmacopeias.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- Peptones included in the composition of the medium favor the excellent growth of colonies.
- Yeast extract is a source of vitamin B complex.
- Starch is a detoxifying agent and also an energy source.
- The heat treatment of blood in the medium lyses red blood cells, releasing growth factors such as hemin (X factor) and NAD (V factor), which are essential for the growth of certain bacteria.
- Isovilatex: Provides additional growth factors and metabolic stimulants that enhance the recovery of fastidious bacteria, including Haemophilus influenzae.
- Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
- Inoculate in order to obtain isolated colonies.
- Incubate at 37 °C, 5 – 10% CO2 for 18 – 24 hours in optimal conditions for the culture of the inoculated germs.
RESULTS
- After incubation for the required duration, observe the colonies growing on the surface of the plate for characteristic traits such as colony size, color, and morphology.
- Identification of the isolated bacteria must be carried out through appropriate subsequent test.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation Conditions | Expected results |
| H. influenzae ATCC 33533 | 35 – 370C, 5 – 10 % CO2, 18 – 24 hours | Good growth |
| S. pneumoniae ATCC 49619 | Weak growth or no growth | |
| N. meningitidis ATCC 13090 | Weak growth or no growth |
For 1 liter of medium
| Polypeptone | 23,0 g |
| Starch | 1,0 g |
| Sodium chloride | 5,0 g |
| Defibrinated horse blood | 50 – 70 mL |
| IsoVitaleX Enrichment | 1 set |
| Agar | 13,5 g |
pH of the ready-to-use medium at 250C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ Chocolate Agar with Isovilatex (CAIS) | 05014 | 10 plates/ box or as per customer request. |
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