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BacterPlate™ Chocolate Agar with VTCN (HP VTCN)
- Ready-to-use medium on 90mm plates supplemented with VTCN for the selective culture of Helicobacter pylori
- Code: 05012
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Chocolate Agar with VTCN (HP VTCN) is selective culture medium designed for the isolation and cultivation of Helicobacter pylori. It is supplemented with Vancomycin, Trimethoprim, Colistin, and Nystatin to suppress the growth of Gram-positive bacteria, Gram-negative bacteria, and fungi, ensuring optimal selectivity for H. pylori. This medium is widely utilized in clinical diagnostics and microbiological quality control.
- The typical composition of base medium (Columbia Agar) corresponds to that defined in European (EP), United States (UP) and Japanese (JP) Pharmacopeias.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- Peptones included in the composition of the medium favor the excellent growth of colonies.
- Yeast extract is a source of vitamin B complex.
- Starch is a detoxifying agent and also an energy source.
- The heat treatment of blood in the medium lyses red blood cells, releasing growth factors such as hemin (X factor) and NAD (V factor), which are essential for the growth of certain bacteria.
- Vancomycin inhibits Gram-positive bacteria.
- Trimethoprim suppresses Gram-negative bacteria by interfering with folate synthesis.
- Colistin: Targets and inhibits Gram-negative contaminants.
- Nystatin: Prevents fungal growth.
- Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
- Inoculate in order to obtain isolated colonies.
- Incubate at 37 °C, 5 – 10% CO2 for 3 to 5 days in optimal conditions for the culture of the inoculated germs.
RESULTS
- After incubation for the required duration, observe the colonies growing on the surface of the plate for characteristic traits such as colony size, color, and morphology.
- Helicobacter pylori: Small, round, translucent colonies with a smooth appearance, often greyish-white
- Contaminants (Gram-positive, Gram-negative bacteria, or fungi) should be inhibited by the VTAP selective agents.
- Identification of the isolated bacteria must be carried out through appropriate subsequent tests
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation Conditions | Expected results |
| H. pylori ATCC 43504 | 35 – 370C, 5 – 10 % CO2, 3 – 5 days | Growth with colony size 1 – 2 mm |
| E. coli ATCC 35128 | Weak growth or no growth | |
| S. aureus ATCC 25923 | Weak growth or no growth |
For 1 liter of medium
| Polypeptone | 23,0 g |
| Starch | 1,0 g |
| Sodium chloride | 5,0 g |
| Defibrinated horse blood | 50 – 70 mL |
| VTCN supplement | 1 set |
| Agar | 13,5 g |
pH of the ready-to-use medium at 250C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ Chocolate Agar with VTCN (HP VTCN) | 05012 | 10 plates/ box or as per customer request. |
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