BacterPlate™ GC Agar có IsoVitalex

Ready-to-use medium on 90mm plates for testing antibiotic susceptibility of Neisseria gonorrhoeae bacteria.
Product code: 05060
Order code: 060015454
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterPlate™ GC Agar with IsoVitalex is a commonly used medium for performing antibiotic susceptibility testing in clinical practice, recommended by CLSI for testing the antibiotic susceptibility of N. gonorrhoeae.

PRINCIPLES

BacterPlate™ GC Agar with IsoVitalex has a nutritional composition that includes:

Peptone, an enzymatic digest of meat and vegetables.
Starch, acting as a protective colloid against toxic substances in the medium.
Phosphate buffer, to prevent pH changes caused by amine production that could affect microbial viability.
Agar, which serves as the solidifying agent for the medium.
The IsoVitalex supplement is added to enhance the growth of gonorrhoeae.

Allow the plates and antibiotic discs to equilibrate to room temperature before use.
Prepare a bacterial suspension:
Select isolated colonies from an agar plate incubated for 18–24 hours (using non-selective medium) and suspend the microorganisms in saline solution. Adjust the turbidity of the suspension to match the 0.5 McFarland standard.
Within 15 minutes after preparing the suspension, dip a sterile swab into the bacterial suspension, gently press and rotate the swab above the liquid level in the tube to remove any excess fluid absorbed by the swab.
Streak the swab evenly over the entire surface of the agar plate, then rotate the plate 60° for the second and third streaking directions.
Allow the agar plate to air-dry naturally in the incubator for at least 3 minutes but no longer than 15 minutes, ensuring that the inoculum is absorbed into the surface of the agar before placing the antibiotic discs.
Using sterile forceps, place the individual antibiotic discs onto the surface of the agar plate.
Do not place more than 5 discs on a 90 mm agar plate. Avoid moving or repositioning the discs once placed, as the diffusion effect of the antibiotic occurs almost immediately. Gently press each disc with a sterile inoculating loop to ensure full contact with the agar surface.
Within 15 minutes after placing the antibiotic discs, incubate the agar plate at 35–37°C for 20–24 hours with 5% CO2 (do not exceed 37°C). Invert the plate during incubation.
Refer to CLSI M45 for incubation time, temperature, and atmospheric conditions for fastidious organisms.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
N. gonorrhoeae ATCC 49226	20 – 24 hours, 35^oC, 5 % CO₂	Good growth, inhibition zone sizes according to CLSI.

For 1 liter of medium

Peptone, special	15,0 g
Corn Starch	1,0 g
Dipotassium hydrogen phosphate	4,0 g
Potassium dihydrogen phosphate	1,0 g
Sodium chloride	5,0 g
IsoVitaleX Enrichment	1 vial
Agar	13,0 g

pH of the ready-to-use medium at 25°C: 7,2 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Product code	Order code	Packaging
BacterPlate™ GC Agar with IsoVitalex	05060	060015454	10 plates/ box or as per customer request.