BacterPlate™ MacConkey Agar (MC)
- Ready-to-use medium on 90mm plates is a selective medium for isolating easily cultured Gram-negative bacteria.
- Code: 05018
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
INTENDED USE
- BacterPlate™ MacConkey Agar is a selective medium for the isolation of enterobacteria in water, food, pharmaceutical products and biological samples of animal origin and in cosmetic products.
- The typical composition corresponds to that defined in the EU Pharmacopeia and in the standards NF EN ISO 21567 and NF EN ISO 21150.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- Bile salts and crystal violet inhibit the growth of Gram-positive bacteria. The dye inhibits primarily the development of enterococci and staphylococci.
- The fermentation of lactose to acid is revealed in the presence of neutral red by the formation of red or pink colonies.
- Lactose-negative colonies form colorless colonies.
1.Detection of Escherichia coli (harmonized chapters of the Pharmacopieae)
- Dry the plates in an incubator, covers partially removed.
- Inoculate the enrichment broth (MacConkey broth) onto a BacterPlate™ MacConkey Agar.
- Incubate at 30 – 35°C for 18 to 72 hours.
- The presence of colonies on the plates requires the completion of the confirmation tests for Escherichia coli.
2.Detection of Escherichia coli (Cosmetics, NF EN ISO 21150)
- Dry the plates in an incubator, covers partially removed.
- Inoculate the enrichment broth onto a BacterPlate™ MacConkey Agar
- Incubate at 30 – 35°C for 24 to 48 hours.
- The presence of brick-red colonies, with or without a zone of precipitated bile, requires the completion of confirmation tests for Escherichia coli
3.Detection of Shigella spp (Food microbiology, NF EN ISO 21567)
- Dry the plates in an incubator, covers partially removed.
- Inoculate the enrichment broth (for Shigella) onto a BacterPlate™ MacConkey Agar
- Incubate at 37 ± 1°C for 20 to 24 hours.
- If characteristic colonies are present, proceed with the necessary confirmation
RESULTS
- Lactose-positive colonies form red colonies, surrounded by a halo due to bile salt precipitation. Lactose-negative colonies are colorless.
- Escherichia coli forms brick-red colonies, with or without a surrounding bile salt precipitate.
- Shigella sonnei are colorless to pale pink and translucid.
- Other Shigella spp. are colorless and translucid.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains
Microbial Standard and Incubation Conditions | Standard (PR) |
Incubation conditions after 18 – 24 hours, at 35 – 37°C, with inoculum ≤ 102 CFU/ mL | |
E. coli ATCC 35218 | ≥ 50 % |
Incubation conditions after 24 – 48 hours, at 35 – 37°C, with inoculum ≤ 103 – 104 CFU/ mL | |
S. aureus ATCC 25923 | Weak growth |
S. flexneri ATCC 12022 | Inhibited, no growth |
For 1 liter of medium
Pancreatic digest of gelatin | 17,0 g |
Tryptone | 1,5 g |
Peptic digest of meat | 1,5 g |
Lactose | 10,0 g |
Bile salts | 1,5 g |
Sodium chloride | 5,0 g |
Neutral red | 30,0 mg |
Crystal violet | 1,0 mg |
Agar | 13,5 g |
pH of the ready-to-use medium at 25°C: 7,0 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
Product Name | Code | Packaging |
BacterPlate™ MacConkey Agar (MC) | 05018 | 10 plates/ box or as per customer request. |
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