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BacterPlate™ Pylori Agar
- Ready-to-use medium on 90mm plates for culturing and screening Helicobacter pylori in gastric biopsy samples.
- Code: 05058
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Pylori Agar is a culture medium designed to screen for Helicobacter pylori in gastric biopsy samples.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- Present in small quantity, the nutritive elements permit the development of stressed microorganisms. The combination of mineral salts maintains the osmotic balance. The presence of sodium pyruvate activates the bacterial metabolism.
- Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
- Streak samples onto the plates
- Incubate under microaerobic conditions at 35 – 37°C for 4 – 7 days.
RESULTS
- After incubation, observe the bacterial colonies growing on the surface of the plate.
- Helicobacter Pylori: Good growth, small, colorless colonies
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| H. pylori ATCC 43504 | 4 – 7 days at 35 – 37 °C, microaerobic | Good growth, small, colorless colonies |
| E. coli ATCC 35218 | 35 – 37 °C, 24 hrs, O2 | No growth |
| C. albican ATCC 10231 | 20 – 30°C, 72 hrs | No growth |
For 1 liter of medium
| Brain infusion solid | 12,5 g |
| Beef heart infusion solids | 5,0 g |
| Proteose peptone | 10,0 g |
| Starch | 2,0 g |
| Glucose | 2,0 g |
| Sodium Chloride | 5,0 g |
| Disodium phosphate | 2,5 g |
| Agar | 10,0 g |
| Defibrinized horse blood | 50 – 100 mL |
| Helicobacter Pylori Selective Supplement | 2 vial |
| Vitox | 2 vial |
pH of the ready-to-use medium at 25°C: 7,4 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ Pylori Agar | 05058 | 10 plates/ box or as per customer request. |
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