avt-BacterPlate™-Môi-trường-2-ngăn-BA-MC

BacterPlate™ Two-Compartment Medium BA/MC

Ready-to-use medium in 90 mm Petri dishes for culturing and isolating pathogenic bacteria from clinical specimens.
Code: 05044
Packaging: 10 plates/box or as per customer’s request.
Storage conditions: 2 – 8°C.
Transportation: Ambient temperature.
Shelf life: 3 months from the date of manufacture.

INTENDED USE

BacterPlate™ Two-Compartment Medium BA/MC is used for the isolation and differentiation of bacteria, particularly Gram-negative bacteria, as well as bacteria capable of hemolysis or lactose fermentation. This medium is applied in microbiological research, clinical diagnostics, and microbiological quality control of water, food, pharmaceuticals, and animal-derived biological samples.

The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BA (Blood Agar) Compartment: This is a blood-based culture medium that helps differentiate bacteria based on their hemolytic properties. Hemolytic reactions can be categorized into three types: beta (β) hemolysis, alpha (α) hemolysis, and gamma (ɤ) hemolysis. These hemolytic reactions help identify the characteristics of bacteria such as Streptococcus and Listeria.

MC (MacConkey Agar) Compartment: MacConkey Agar is a selective medium used to isolate Gram-negative bacteria, particularly enteric bacteria. Bacteria capable of lactose fermentation will form red or pink colonies, while non-lactose fermenters will form colorless or transparent colonies

INSTRUCTIONS FOR USE

Allow the agar plates to reach room temperature before use.
Dry the agar plates in an incubator with the lid slightly open.
Inoculate the sample by spreading it evenly on the surface of the agar using a sterile inoculating loop.
Incubate at 35–37°C for 18–24 hours.

RESULTS

BA Compartment:
- Beta Hemolysis (β): Colonies will appear with a complete hemolytic zone (clear zone) around them. Beta-hemolytic bacteria include Streptococcus pyogenes and Listeria.
- Alpha Hemolysis (α): Colonies will have a narrow, greenish or faint hemolytic zone. Alpha-hemolytic bacteria include Streptococcus pneumoniae.
- Gamma Hemolysis (ɤ): No hemolytic zone, e.g., Enterococcus faecalis.
MC Compartment:
- Lactose Positive: Lactose-fermenting bacteria will form red colonies, surrounded by a precipitate of bile salts.
- Lactose Negative: Non-lactose fermenting bacteria will form colorless or transparent colonies.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
BA Compartment
S. pneumonia ATCC 49619	Incubate at 35–37°C for 18–24 hours.	Good growth, α hemolysis.
S. pyogenes ATCC 19615		Good growth, hemolysis.
E. coli ATCC 25922		Good growth
S. aureus ATCC 25923		Good growth.
MC Compartment
E. coli ATCC 25922	Incubate at 35–37°C for 18–24 hours.	Good growth
S. aureus ATCC 25923		Weak growth
S. flexneri ATCC 12022		Inhibited, no growth

For 1 liter of BA medium

Polypeptone	23,0 g
Starch	1,0 g
Sodium chloride	5,0 g
Defibrinated sheep blood	50 – 70 mL
Agar	13,5 g

pH of the ready-to-use medium at 25°C: 7,3 ± 0,2

For 1 liter of MC medium

Pancreatic digest of gelatin	17,0 g
Tryptone	1,5 g
Peptic digest of meat	1,5 g
Lactose	10,0 g
Bile salts	1,5 g
Sodium chloride	5,0 g
Neutral red	30,0 mg
Crystal violet	1,0 mg
Agar	13,5 g